FastQCFastQC Report
Mon 17 Apr 2023
000000000-KWF5G_l01_n02_ELP3F12.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-KWF5G_l01_n02_ELP3F12.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5692579
Sequences flagged as poor quality0
Sequence length301
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGAGTAACCGCAACATAGTGAAAACACAGTTATCCATACTAGTTAGAGAA501814188.15232955045508No Hit
TGCGTAACCGCAACATAGTGAAAACACAGTTATCCATACTAGTTAGAGAA1092831.9197449872895924No Hit
TGAGTAACCGCAACATAGTGAAAACACAGTTATCCATACTAGTGAGAGAA343790.6039266209568633No Hit
TGGGTAACCGCAACATAGTGAAAACACAGTTATCCATACTAGTTAGAGAA340050.5973566638249552No Hit
TGAGTAACCGCAACATAGTGAAAACACAGTTAGCCATACTAGTTAGAGAA159370.2799609807786594No Hit
TGAGTAACCGCAACATAGTGAAAACACAGTTATCAATACTAGTTAGAGAA139080.24431808500154323No Hit
TGAGTAACCGCAACATAGTGAAAACACAGTTATCCATACGAGTTAGAGAA126880.2228866740364956No Hit
TGAGTAACCGCAACATAGTGAAAACACAGTTATCCATACTCGTTAGAGAA106410.1869275771139935No Hit
TGAGTAACCGCAACATAGTGAAAACACAGTTATCCATACGAGTGAGAGAA84940.14921180716156945No Hit
TGAGTAACCGCAACATAGTGAACACACAGTTATCCATACTAGTTAGAGAA82360.14467959074437087No Hit
TGAGTAACCGCAACATAGTGAAAACACAGTTATCCATACTAGGTAGAGAA79130.139005536857723No Hit
TGAGTAACCGCAACATAGTGAAAACACAGTTAGCCATACTAGTGAGAGAA78530.13795153303976987No Hit
TGAGTAACCGCAACATAGTGAAAACACAGGTATCCATACTAGTTAGAGAA77890.1368272623006198No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)