FastQCFastQC Report
Mon 17 Apr 2023
000000000-KWF5G_l01_n02_ELP3E8.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-KWF5G_l01_n02_ELP3E8.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4787
Sequences flagged as poor quality0
Sequence length301
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGAGTAACCGCAACATAGTGAAAACACAGTTATCCATACTAGTTAGAGAA387380.90662210152496No Hit
TGCGTAACCGCAACATAGTGAAAACACAGTTATCCATACTAGTTAGAGAA982.047211196991853No Hit
AAGGCCACGTCATCCCCACCTTCCTCCGGTTTGTCACCGGCAGTCACCTT430.8982661374556089No Hit
TGAGTAACCGCAACATAGTGAAAACACAGTTATCCATACTAGTGAGAGAA220.4595780238144976No Hit
TGAGTAACCGCAACATAGTGAAAACACAGTTAGCCATACTAGTTAGAGAA90.1880091915604763No Hit
TGAGTAACCGCAACATAGTGAAAACACAGTTATCCATACTCGTTAGAGAA90.1880091915604763No Hit
AAGGCCACGTCATCCCCACCTTCCTCCGGTTTGTCACCGGCAGTCTCCTT90.1880091915604763No Hit
TGAGTAACCGCAACATAGTGAAAACACAGTTATCCATACGAGTTAGAGAA80.16711928138709004No Hit
GGAGTAACCGCAACATAGTGAAAACACAGTTATCCATACTAGTTAGAGAA80.16711928138709004No Hit
TGAGTAACCGCAACATAGTGAAAACACAGTTATCAATACTAGTTAGAGAA60.1253394610403175No Hit
TGAGTAACCGCACATAGTGAAAACACAGTTATCCATACTAGTTAGAGAAC60.1253394610403175No Hit
TGAGTAACCGCAACATAGTGAAAACACAGTTATCCATACGAGTGAGAGAA50.10444955086693128No Hit
TGAGTAACCGCAACATAGTGAACACACAGTTATCCATACTAGTTAGAGAA50.10444955086693128No Hit
TGAGTAACCGCAACATAGTGAAAACACAGGTATCCATACTAGTTAGAGAA50.10444955086693128No Hit
GGAGTAACCGCAACATAGTGAAAACACAGTTATCCATACGAGTGAGAGAA50.10444955086693128No Hit
TGAGTAACCGCAACAGAGTGAAAACACAGTTATCCATACTAGTTAGAGAA50.10444955086693128No Hit
TGAGTAACCGCAACATAGTGAAAACACAGGTATCCATACTAGTGAGAGAA50.10444955086693128No Hit
GGAGTAACCGCAACAGAGTGAAAACACAGTTATCCATACTAGTGAGAGAA50.10444955086693128No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)