FastQCFastQC Report
Mon 17 Apr 2023
000000000-KWF5G_l01_n02_ELP3E5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-KWF5G_l01_n02_ELP3E5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences182
Sequences flagged as poor quality0
Sequence length301
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGAGTAACCGCAACATAGTGAAAACACAGTTATCCATACTAGTTAGAGAA3519.230769230769234No Hit
AAGGCCACGTCATCCCCACCTTCCTCCGGTTTGTCACCGGCAGTCTCCTT147.6923076923076925No Hit
AAGGCCACGTCATCCCCACCTTCCTCCGGTTTGTCACCGGCAGTCACCTT31.6483516483516485No Hit
AAGGCCACGTCATCCCCACCTTCCTCCGGTTTGTCACCGGAAGTCTCCTT21.098901098901099No Hit
TGCGTAACCGCAACATAGTGAAAACACAGTTATCCATACTAGTTAGAGAA21.098901098901099No Hit
TGAGTAACCGCAACATAGTGAAAACACAGTTATCCATACTAGTTAGAGCA21.098901098901099No Hit
AAGGCCACGTCATCACCACCTTCCTCCGGTTTGTCACCGGCAGTCTCCTT21.098901098901099No Hit
AAGGCCACGTCATCCCCACCTTCCTCCGGTTTGTCACAGGCAGTCTCCTT21.098901098901099No Hit
AAGGTCACGGCATCCTCACCTACCTCCGGTTTTTCATCGTCAGTCTCCAT10.5494505494505495No Hit
TAAGTAACCTCAACCTCGCGTACACACAGTTTGCCACCCTAGTTCGCCAT10.5494505494505495No Hit
AAGGCCACCTCATCCCCACCTTCCTCCGGTTTTTCACCGTCATTCTCCTT10.5494505494505495No Hit
TAGGCAACGTCATCCTCACGTTCCTCCAGTTTTTCACCCTAGTTCTCCAT10.5494505494505495No Hit
AAGGCCACGTCATCCCCACCTTCCTCCGGTTTGCCACCGTCAGTCTCCTT10.5494505494505495No Hit
AAGGCCACGTCATCCTCACCTTCCCACGGTTTTTCACCGGCAGTCTCCTT10.5494505494505495No Hit
TAGGTCACCTCAACCTAGTGAACACCCGGTTATCCATCCTAAGTAGCGAT10.5494505494505495No Hit
AAGGCCACGTCATCCCCACCTTCCTCCGGTTTGTCACCCGCAGTCTCCTT10.5494505494505495No Hit
TGAGCCACCGCATCCTCACCATCCCACAGTTATTCATCCTCGTTCGCGTT10.5494505494505495No Hit
TAAGCCACGTCAACCCCGCGATCCCCCGGTTATTCATCCTCGTTCTCGTT10.5494505494505495No Hit
TAGGCAACGTCATCCCCACCTTCCTCCGGTTTGTCACCCTCAGTCTCCTT10.5494505494505495No Hit
AGGGCAACCTCATCCTCACGTACACACAGTTATCCACACTCGTTCTCGTT10.5494505494505495No Hit
AAGGCCACGTCATCCCCACCTTCCTCAGGTTTGTCACCGGCAGTCTCCTT10.5494505494505495No Hit
TGAGTAACCGCAACATAGAGAAAACACAGTGAGCCATACGAGGGAGAAAA10.5494505494505495No Hit
AAGGCCACGTCATCCCCACCTTCCTCCGGTTTGTCACCGGCAGTCTACTT10.5494505494505495No Hit
TAAGTCACCTCAACCTCGTGAAAACACGGTTATCCATACTCGTTCGAGTA10.5494505494505495No Hit
CAGGCCACGTCATCCCCACCTTCCTCCCGTTTGTCACCGCCAGTCTCCTT10.5494505494505495No Hit
AAGGCCACGTCATCCTCACCTTCCTACGGTTTGTCATCCTCATTCTCATT10.5494505494505495No Hit
TGGGTCACGGCATCCTCACCTTCCCCCGGTTATCCATCGTCGTTCTACTA10.5494505494505495No Hit
TAGGCCACGGCATCCCCATCTTCCTCCGGTTTTTCACCCTCAGTATCGTT10.5494505494505495No Hit
TGAGTAACCTCAACATAGTGATACCACAGTTATCCATACTAGTTCGCGAA10.5494505494505495No Hit
TAGGTAACCGCATCATCATCTTCCCCCGGTTTTCCATCCTCATTCTCGTT10.5494505494505495No Hit
AAGGCCACGTCATCCCCACCTTCATCCGGTTTGTCACCGGAAGTCTCCTT10.5494505494505495No Hit
TAGGTCACCGCATCATCGTCTTACCACGGTTATTCATCCTCATTATCCAT10.5494505494505495No Hit
AAGGCCACGTCATCCCCACCTTCCTCCGGTTTGTCACCGGCGGTCGCCAT10.5494505494505495No Hit
TGAGTCACCTCAACCTCACCTTCCCCCAGTTATTCATCCTCATTCGCCTA10.5494505494505495No Hit
TAGGTCACCTCATCCTCATCTCCCCCCGGTTTTCCATACTCGTTCGCGAT10.5494505494505495No Hit
AACGCAACGTCATCCCCACCTTCCTCCGGTTTTTCACCCGCATTCTCCTT10.5494505494505495No Hit
TGGGTAACCTCATCCTAGTGTACATACAGTTATTCACACGAGTTAGACTA10.5494505494505495No Hit
AAGGCAACGTCATCCCCACGTTCATACGGTTTGTCACCGGCAGTCGCCTT10.5494505494505495No Hit
AAGGCCACGTCATCCCCACCTTCCTCCGGTTTTTCACCGTCAGTCGCCTT10.5494505494505495No Hit
TGAGTAACCGCAACATAGTGAAAACACAGTTAGCCATACTAGGTAGAGAA10.5494505494505495No Hit
TAAGCCACGTCATCACCACCTTCCTCCGGTTATTCACCCTAATTATCCTT10.5494505494505495No Hit
TGAGTAACCTCATCATAGTGTAACCACAGTTATCCATACTCGTTAGAGAT10.5494505494505495No Hit
AAGGCCACGTCATCACCACCTTCCTACGGTTAGTCACCGTCGTTATCCTT10.5494505494505495No Hit
TGAGTAACCGCAACCTAGTGATCACACAGTTATCCATACTCGTTAGCGAA10.5494505494505495No Hit
TGAGCAACCTCAACACCATCTTCCTACAGTTTGTCATACGCGGTCTCCTT10.5494505494505495No Hit
AAGGCCACCTCATCCCCACCTTCCTCCAGTTTGTCACCCTCATTCTCCTT10.5494505494505495No Hit
TAGGTAACGTCATCCCAGCCTTCACACGGTTTTCCACCCGCATTATCCTT10.5494505494505495No Hit
TGAGTCACCGCAACATAACGAAAACCCAGTTATCCATACTAATTCGCCAA10.5494505494505495No Hit
AAGGCCACGTCATCCCAACCTTCCTACGGTTTGTCATAGGAAGTCTCCTT10.5494505494505495No Hit
TGAGTAACCTCAACCTAGTCTTCACACGGTTATCCATCCTCGTTCTCCAA10.5494505494505495No Hit
AAGGCCACGTCATCCCCACCTTCCTCCGGTTTGTCACCGTCATTCACCTT10.5494505494505495No Hit
TGAGCCACCGCAACACCACCTTACTCCAGTTTTTCATCCTCATTCGCCAT10.5494505494505495No Hit
TAGGCCACGTCATCCCCACCTTCCTCCGGTTTTTCACCGTCAGTCTCCTT10.5494505494505495No Hit
TAGGTCACCTCAACCCCGCCTTCCCCCAGTTATCCATCCTCGGTCGCCAT10.5494505494505495No Hit
AAAGTCACGTCATCCCCACCTTCCTCCGGTTTGTCATCGTCAGTCTCCTT10.5494505494505495No Hit
TGAGTAACCGCAACATAGTGAAAACTCAGTTATCCATACTAGTTAGAGAA10.5494505494505495No Hit
AAGGCCACGTCATCCCCACCTTCCTCCAGTTTGTCACCGTCATTCTCCTT10.5494505494505495No Hit
TAGGTCACCTCATCCCCGCCTTCATCCGGTTATCCATCGTCGGTCTCCTT10.5494505494505495No Hit
AAGGCAACGTCATCACCACCTTCCTCCGGTTTGTCACCGGCAGTCTCCTT10.5494505494505495No Hit
AAGGCCACGTCCTCCCCACCTTCCTCCGGTTTGTCACCGGCAGTCTCCTT10.5494505494505495No Hit
AAGGCCACGTCATCATCACCTTCCTCCGGTTTGTCATCGGCAGTCTCCTT10.5494505494505495No Hit
TAGGCCACCTCATCATAGCCTACATCCGGTTTTTCATACTCGGTCGCCTT10.5494505494505495No Hit
AAGTCCACGTCATCCCCACCTTCCTCCGGTTTGTCACCGGCAGTCTCCTT10.5494505494505495No Hit
TAAGTCACCTCAACATCATGAAAACCCGGTTATCCATCCTCATTCGAGAT10.5494505494505495No Hit
AAAGTCACCGCATCCTCATCTTCACACAGTTAGCCATACTCATTCGACAT10.5494505494505495No Hit
TGAGTCACCGCATCATAGTGATCCTACAGTTATCCATACGAGTTAGAGTA10.5494505494505495No Hit
AAGGCCACCTCATCCCCACCTTCCTACGGTTTGTCACCCGCAGTCTCCTT10.5494505494505495No Hit
AAGGTCACCTCATCCTCACCTTCCTCCGGTTATTCATACTCGTTCTCCTT10.5494505494505495No Hit
TAGGCCACCTCATCATAGCCTACCCACAGTTAGTCATCGTAGTTCGCGTA10.5494505494505495No Hit
TGGGTCACCTCATCATAGTCATAACACAGTTATTCATACTAGTTCGAGTT10.5494505494505495No Hit
TGGGTCACCGCAACATCGTGAACACGCAGTTATCCATCCTCGTTCGCCAT10.5494505494505495No Hit
TGAGTAACCTCATCATAGTGTTCACCCAGTTATCCATACTCGTTCTCCAT10.5494505494505495No Hit
AAGGCCACGTCATCCTCACCTTCCTCCGGTTTTTCACCGTCATTCTCCTT10.5494505494505495No Hit
AAGGCCACGTCATCCCCACCTTCCTCCGGTTTGTCACCGTCAGTCTCCTT10.5494505494505495No Hit
AAGGCCACGTCATCCCCACCTTCCTACGGTTTGTCACCCGCAGTCTCCTT10.5494505494505495No Hit
TGAGTACCCGCAACCTAGTGAAAACACCGTTATCCATACTCGTTAGAGAA10.5494505494505495No Hit
AAAGCAACGTCAACATAGCGTTCATCCAGTTTTTCACACTCGGTCGCCAT10.5494505494505495No Hit
TGGGTCACCTCATCCTCGTGTTACCCCGGTTTTCCATCCTCGTTCTCCTT10.5494505494505495No Hit
AAGGCCACGTCATCCTAGCCTTCCTCCAGTTTGTCACCGTAATTCGACTT10.5494505494505495No Hit
TGAGTAACCGCAACATAGTGAAAACACAGTTTTCCATACTAGTTAGAGAA10.5494505494505495No Hit
TGAGTAACCGCATCATCGCCTTCCCCCGGTTTTCCATCCTCGTTAGACAT10.5494505494505495No Hit
AAGGCCACGTCAACCCCACCTTCCTCCGGTTAGTCACCGGCAGTCGCCTT10.5494505494505495No Hit
TAAGTAACCGCAACCTCGTCTACACACAGTTAGCCATACTCGTTAGCGTA10.5494505494505495No Hit
TAAGTAACCGCAACCTCGTGTACATACGGTTTTCCATACTCGTTCTCGTA10.5494505494505495No Hit
TAGGTCACCTCATCATCATCTACCCACAGTTTTCCATACTAGTTCTCCAA10.5494505494505495No Hit
AGGGCCACGTCATCCTCACCTTCACACGGTTATCCATCCGCGTTCTCGAT10.5494505494505495No Hit
TAAGTCACCGCAACCTCGTCTACACACAGTTATCCATACTAGTTAGCGAT10.5494505494505495No Hit
TGAGCAACCTCAACATCGCCAACCTACAGTTTTTCATCGTCGTTCGCGTA10.5494505494505495No Hit
AAGGCCACGTCATCCCCACCTTCCTCCGGTTTGTCATAGGCAGTCTCCTT10.5494505494505495No Hit
TGAGTAACCTCAACCTCGTCTACCCACAGTTATCCATACGCATTCTACAT10.5494505494505495No Hit
TAGGTCACCTCATCATAACCTACACCCGGTTATTCATCCTCGTTCGCGTT10.5494505494505495No Hit
AAGGCCACGTCATCACCACCTTCCTACGGTTTTTCACCGTCGTTCTACAT10.5494505494505495No Hit
TAGGTCACCGCAACATCATCTTCCCCCGGTTATTCATCCTCATTAGCGAT10.5494505494505495No Hit
AAGGCCACGTCATCCCCACCTTCCTCCGGTTATTCACCCTCAGTCTCCTT10.5494505494505495No Hit
TGAGCCACCTCAACATCGCCTAACTCCAGTTTGCCATCCTAGTTATCCTA10.5494505494505495No Hit
AAGGCCACCTCATCACCGCCTTCCTCCGGTTTTTCACACGCGTTATCCTT10.5494505494505495No Hit
TGCGTAACCGCAACATAGTGAAAACACAGTTATCCATACTAGATAGAGCA10.5494505494505495No Hit
AAAGTCACCTCATCCTCATCTACCCCCGGTTTTCCACACTCATTCTCCTT10.5494505494505495No Hit
TAGGTCACGTCATCCTCACCTTCATCCGGTTATTCATCGTCGTTCTCCTT10.5494505494505495No Hit
AAGGTCACGTCATCCTCACCTTCCCACGGTTAGTCACACTAAGTCTCCTT10.5494505494505495No Hit
AAGGCCACGTCATCCCCTCCTTCCTCCGGTTTGTCACCGGCAGTCTCCTT10.5494505494505495No Hit
AGAGCAACCGCAACATAGTGTAAACACAGTTATCCATACTAGTTAGAGAT10.5494505494505495No Hit
AAGGCCACGTCATCCTCACCTTCCCACGGTTATTCATACGCATTCTCCTT10.5494505494505495No Hit
AAGGCCACGTCATCCCCACCTTACTCCGGTTTGTCACCGGCAGTCTCCTT10.5494505494505495No Hit
AAAGCCACGTCATCCCCACCTTCCTACGGTTTGTCACCGGCAGTCTCCTT10.5494505494505495No Hit
TAGGCCACGTCATCCCCACCTTCCTCCGGTTTGTCATCGGAATTCTCCTT10.5494505494505495No Hit
AAGGCCACGTCATCCTAGCCTTCCTCCGGTTTGCCATAGGTAGTCTCCTT10.5494505494505495No Hit
AAAGCCACCTCATCCCCACCTTCCTACGGTTATTCATCCTCGGTCGACTT10.5494505494505495No Hit
AGGGCCACGGCATCCCCATCTTCATCCAGTTTTTCACCCTCGTTCGCCAA10.5494505494505495No Hit
TGAGTAACCTCAACCTCGTCAAAACACGGTTATCCATACTCGTTAGAGAA10.5494505494505495No Hit
TGAGCCACCTCATCCTCGCGTACACCCAGTTAGTCACACTCGTTATCCTA10.5494505494505495No Hit
AAGGTCACCTCATCCTCACCTTCCTCCGGTTAGCCATCGTCAGTCTCCTA10.5494505494505495No Hit
TAGGCCACGTCAACCTCACCATCATCCGGTTTTCCATCCGAATTAGACAT10.5494505494505495No Hit
AACGCCACCTCACCCCCACCCTCCTCCCGTTTTCCACCCGCACTCTCCTT10.5494505494505495No Hit
TGGGTAACGTCATCCTAACCTTCACACGGTTTGTCATACTAAGTCTACTT10.5494505494505495No Hit
AAAGCCACCTCATCCCCACCTTCCTCCGGTTTGTCACCGGCGTTCGCCTT10.5494505494505495No Hit
AAGGCCACGTCATCCTCACCTTCCTCCGGTTATCCATACGCGGTCGCGAT10.5494505494505495No Hit
TAGTAACCGCAACATAGTGAAAACACAGTTATTCATACTAGTTAGAGAAC10.5494505494505495No Hit
TAAGTCACCTCATCCCCACGTACCCCCGGTTATTCATACGCGTTATCGTA10.5494505494505495No Hit
AAGGCCACGTCATCCTCACCTACACACAGTTATTCACACTAATTCGCCTT10.5494505494505495No Hit
AAGGCCACGTCATCCCCACCTTCCTACGGTTTGTCACCGTCAGTCTCCTT10.5494505494505495No Hit
AAGGCCACCTCATCCCCGCCTTCCTCCGGTTATTCACCCTCGGTCTACTA10.5494505494505495No Hit
TAGGTCACGTCATCCCCACCTTCATACGGTTAGTCATCGGAGGTCTCCTT10.5494505494505495No Hit
TGAGCAACCGCATCCTAGCGATCACACAGTTATCCATAGTAGTTATAGAA10.5494505494505495No Hit
TAGGCCACCTCATCCTCACCTTCCTCCGGTTTTTCACCCTCATTCTCCTT10.5494505494505495No Hit
AAGGCCACGTCATCCCCTCCTTCATCCGGTTTGTCACCGGCAGTCTCCTT10.5494505494505495No Hit
TAGGCCACGTCAACATCACCTTCACCCGGTTTTCCATACGAGTTATCCTT10.5494505494505495No Hit
AGAGTAACCGCAACCTAGCGAACACACAGTTATCCATACTAGTTATCGTA10.5494505494505495No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)