FastQCFastQC Report
Mon 17 Apr 2023
000000000-KWF5G_l01_n02_ELP3E12.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-KWF5G_l01_n02_ELP3E12.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6081996
Sequences flagged as poor quality0
Sequence length301
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGAGTAACCGCAACATAGTGAAAACACAGTTATCCATACTAGTTAGAGAA534700987.91536528468615No Hit
TGCGTAACCGCAACATAGTGAAAACACAGTTATCCATACTAGTTAGAGAA1145571.883542836923931No Hit
TGGGTAACCGCAACATAGTGAAAACACAGTTATCCATACTAGTTAGAGAA362600.5961858574060226No Hit
TGAGTAACCGCAACATAGTGAAAACACAGTTATCCATACTAGTGAGAGAA358500.5894446494210125No Hit
TGAGTAACCGCAACATAGTGAAAACACAGTTAGCCATACTAGTTAGAGAA179650.29538000353831206No Hit
TGAGTAACCGCAACATAGTGAAAACACAGTTATCAATACTAGTTAGAGAA152760.25116754433906235No Hit
TGAGTAACCGCAACATAGTGAAAACACAGTTATCCATACGAGTTAGAGAA135450.2227064930657633No Hit
TGAGTAACCGCAACATAGTGAAAACACAGTTATCCATACTCGTTAGAGAA116900.19220663742626598No Hit
TGAGTAACCGCAACATAGTGAACACACAGTTATCCATACTAGTTAGAGAA95240.15659332890057803No Hit
TGAGTAACCGCAACATAGTGAAAACACAGTTATCCATACGAGTGAGAGAA87660.14413031511365676No Hit
TGAGTAACCGCAACATAGTGAAAACACAGTTAGCCATACTAGTGAGAGAA86960.1429793771649965No Hit
TGAGTAACCGCAACATAGTGAAAACACAGTTATCCATACTAGGTAGAGAA83920.13798101807367188No Hit
TGAGTAACCGCAACATAGTGAAAACACAGGTATCCATACTAGTTAGAGAA83400.13712603559752423No Hit
TGGTAACCGCAACATAGTGAAAACACAGTTATCCATACTAGTTAGAGAAC61330.10083860627333525No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)