FastQCFastQC Report
Mon 17 Apr 2023
000000000-KWF5G_l01_n02_ELP3E11.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-KWF5G_l01_n02_ELP3E11.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences422
Sequences flagged as poor quality0
Sequence length301
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGAGTAACCGCAACATAGTGAAAACACAGTTATCCATACTAGTTAGAGAA40395.49763033175356No Hit
TGGTAACCGCAACATAGTGAAAACACAGTTATCCATACTAGTTAGAGAAC20.47393364928909953No Hit
TGAGTAACCGCAACATAGTGAAAACACAGTTATCCATACTCGTTAGAGAA20.47393364928909953No Hit
TGCGTAACCGCAACATAGTGAAAACACAGTTATCCATACTAGTTAGAGAA20.47393364928909953No Hit
TGAGTAACCGCAACATAGTGAAAACACAGTTATCAATACTAGTTAGAGAA10.23696682464454977No Hit
TGAGTAACCGCAACATAGTGAAAACACCGTTATCCCTCCTCGTTCGAGAA10.23696682464454977No Hit
TGAGTAACAGCAACATAGTGAAAATACAGTTATCCATACTAGTTTGAGCA10.23696682464454977No Hit
TAGTTACCCCCACACTAGTAAAACACCATTTTTCCTTCCTATTTAGGGAC10.23696682464454977No Hit
CGAGTAACCGCAACATAGTGAAACCCCCGTTATCCATACCCCTTAGAGCA10.23696682464454977No Hit
TGAGTAACCGCAACATAGTGAAACCACAAATATCAATACTAGTTAGAAAA10.23696682464454977No Hit
TGGTAACCGCAACATAGTGAAAACACAGTTATCCATACTGGTTAGAGAAC10.23696682464454977No Hit
TGAGTAACCGCAACATAGTGAAAACACAGTATCCATACTAGTTAGAGAAC10.23696682464454977No Hit
TGAGTAACCGCAACATAGTGAAAACACAGTTATCCATACTAGTTAGACAA10.23696682464454977No Hit
TGAGTAACCGCAACATCGTGAAAACACAGTTATCCATACTAGTTAGAGAA10.23696682464454977No Hit
GAGTAACCGCAACATAGCGAAAACACAGTTATCCATACTAGTTAGAGAAC10.23696682464454977No Hit
TGAGTAACCGCACCATAGTGAAACCCCAGTTATCAATATTAGTTAGAGAA10.23696682464454977No Hit
TGAGTAACCGCACCATAGTGAAAACACAGTTATCCATACTAGTTAGAGAA10.23696682464454977No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)