FastQCFastQC Report
Mon 17 Apr 2023
000000000-KWF5G_l01_n02_ELP3D7.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-KWF5G_l01_n02_ELP3D7.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4518
Sequences flagged as poor quality0
Sequence length301
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGAGTAACCGCAACATAGTGAAAACACAGTTATCCATACTAGTTAGAGAA409790.68171757414785No Hit
TGCGTAACCGCAACATAGTGAAAACACAGTTATCCATACTAGTTAGAGAA1052.3240371845949537No Hit
AAGGCCACGTCATCCCCACCTTCCTCCGGTTTGTCACCGGCAGTCACCTT420.9296148738379815No Hit
TGGGTAACCGCAACATAGTGAAAACACAGTTATCCATACTAGTTAGAGAA250.5533421868083223No Hit
TGAGTAACCGCAACATAGTGAACACACAGTTATCCATACTAGTTAGAGAA120.2656042496679947No Hit
TGAGTAACCGCAACATAGTGAAAACACAGTTATCCATACTCGTTAGAGAA90.199203187250996No Hit
TGAGTCACCGCAACATAGTGAAAACACAGTTATCCATACTAGTTAGAGAA90.199203187250996No Hit
TGAGTAACCGCAACATAGTGAAAACACAGTTATCCATACTAGTTAGAGCA80.17706949977866313No Hit
TGGTAACCGCAACATAGTGAAAACACAGTTATCCATACTAGTTAGAGAAC80.17706949977866313No Hit
TGAGTAACCGCAACATAGTGAAAACACAGTTATCTATACTAGTTAGAGAA70.15493581230633025No Hit
TGAGTAACCGCAACATAGTGAAAACACAGTTATCCATACTAGTTAGCGAA60.13280212483399734No Hit
TGAGTAACCGCAACATAGTGAAAACACAGTTATCAATACTAGTTAGAGAA60.13280212483399734No Hit
TTAGTAACCGCAACATAGTGAAAACACAGTTATCCATACTAGTTAGAGAA60.13280212483399734No Hit
TGAGTAACCGCAACATAGTGAAATCACAGTTATCCATACTAGTTAGAGAA50.11066843736166444No Hit
TGAGTAACCGCAACATAGTGAAAACACAGTTATCGATACTAGTTAGAGAA50.11066843736166444No Hit
TGATAACCGCAACATAGTGAAAACACAGTTATCCATACTAGTTAGAGAAC50.11066843736166444No Hit
TGAGTAACCGCCACATAGTGAAAACACAGTTATCCATACTAGTTAGAGAA50.11066843736166444No Hit
TGAGTAACCGCAACATAGTGAAAACACAGTTATCCTTACTAGTTAGAGAA50.11066843736166444No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)