FastQCFastQC Report
Mon 17 Apr 2023
000000000-KWF5G_l01_n02_ELP3C6.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-KWF5G_l01_n02_ELP3C6.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6375
Sequences flagged as poor quality0
Sequence length301
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGAGTAACCGCAACATAGTGAAAACACAGTTATCCATACTAGTTAGAGAA582991.43529411764706No Hit
TGCGTAACCGCAACATAGTGAAAACACAGTTATCCATACTAGTTAGAGAA1892.9647058823529413No Hit
TGAGTAACCGCAACATAGTGAAAACACAGTTATCCATACTCGTTAGAGAA180.2823529411764706No Hit
TGAGTAACCGCAACATAGTGAAAACACAGTTATCAATACTAGTTAGAGAA160.25098039215686274No Hit
TGAGTAACCGCAACATAGTGAACACACAGTTATCCATACTAGTTAGAGAA150.2352941176470588No Hit
TGAGTCACCGCAACATAGTGAAAACACAGTTATCCATACTAGTTAGAGAA150.2352941176470588No Hit
TGAGTAACCGCAACATAGTGAAAACACAGTTATCCATACTAGTTAGAGCA100.1568627450980392No Hit
TGAGTAACCGCAACATAGTGCAAACACAGTTATCCATACTAGTTAGAGAA100.1568627450980392No Hit
TGAGTAACCGCAACATAGTGACAACACAGTTATCCATACTAGTTAGAGAA100.1568627450980392No Hit
TGAGTAACCGCAACATAGTGAAAACACAGTTATCCATACTAGTTAGCGAA100.1568627450980392No Hit
TGGGTAACCGCAACATAGTGAAAACACAGTTATCCATACTAGTTAGAGAA100.1568627450980392No Hit
TGGTAACCGCAACATAGTGAAAACACAGTTATCCATACTAGTTAGAGAAC90.1411764705882353No Hit
TGAGTAACCGCACATAGTGAAAACACAGTTATCCATACTAGTTAGAGAAC80.12549019607843137No Hit
TGAGTAACCGCCACATAGTGAAAACACAGTTATCCATACTAGTTAGAGAA80.12549019607843137No Hit
TGAGTAACCGCAACATAGTTAAAACACAGTTATCCATACTAGTTAGAGAA70.10980392156862745No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)