FastQCFastQC Report
Mon 17 Apr 2023
000000000-KWF5G_l01_n02_ELP3C3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-KWF5G_l01_n02_ELP3C3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5222
Sequences flagged as poor quality0
Sequence length301
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGAGTAACCGCAACATAGTGAAAACACAGTTATCCATACTAGTTAGAGAA480091.91880505553428No Hit
TGCGTAACCGCAACATAGTGAAAACACAGTTATCCATACTAGTTAGAGAA981.876675603217158No Hit
TGGGTAACCGCAACATAGTGAAAACACAGTTATCCATACTAGTTAGAGAA230.4404442742244351No Hit
TGAGTAACCGCAACATAGTGAACACACAGTTATCCATACTAGTTAGAGAA220.42129452317119875No Hit
TGGTAACCGCAACATAGTGAAAACACAGTTATCCATACTAGTTAGAGAAC120.2297970126388357No Hit
TGAGTAACCGCAACATAGTGAAAACACAGTTATCAATACTAGTTAGAGAA110.21064726158559938No Hit
TGAGTAACCGCAACATAGTGAAAACACAGTTATCCATACTAGTTAGAGCA90.17234775947912676No Hit
TGAGTAACCGCAACATAGTGAAAACACAGTTATCCATACTCGTTAGAGAA90.17234775947912676No Hit
TGAGTAACCGCAACATAGTGAAAACACAGTTATCCATACTAGTTAGCGAA90.17234775947912676No Hit
TGAGTACCGCAACATAGTGAAAACACAGTTATCCATACTAGTTAGAGAAC60.11489850631941785No Hit
TGAGTAACTGCAACATAGTGAAAACACAGTTATCCATACTAGTTAGAGAA60.11489850631941785No Hit
TGAGTAACCGCACATAGTGAAAACACAGTTATCCATACTAGTTAGAGAAC60.11489850631941785No Hit
TGAGTAACCGCAACATAGTGAAACCACAGTTATCCATACTAGTTAGAGAA60.11489850631941785No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)