FastQCFastQC Report
Mon 17 Apr 2023
000000000-KWF5G_l01_n02_ELP3B5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-KWF5G_l01_n02_ELP3B5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6220
Sequences flagged as poor quality0
Sequence length301
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGAGTAACCGCAACATAGTGAAAACACAGTTATCCATACTAGTTAGAGAA542787.2508038585209No Hit
TGCGTAACCGCAACATAGTGAAAACACAGTTATCCATACTAGTTAGAGAA1442.315112540192926No Hit
TGGGTAACCGCAACATAGTGAAAACACAGTTATCCATACTAGTTAGAGAA280.45016077170418006No Hit
TGAGTAACCGCAACATAGTGAAAACACAGTTATCCATACTAGTGAGAGAA230.36977491961414793No Hit
TGAGTAACCGCAACATAGTGAAAACACAGTTAGCCATACTAGTTAGAGAA230.36977491961414793No Hit
TGAGTAACCGCAACATAGTGAAAACACAGTTATCCATACGAGTTAGAGAA150.2411575562700965No Hit
TGAGTAACCGCAACATAGTGAAAACACAGTTATCCATACTCGTTAGAGAA140.22508038585209003No Hit
TGAGTAACCGCAACATAGTGAACACACAGTTATCCATACTAGTTAGAGAA130.2090032154340836No Hit
TGAGTAACCGCAACATAGTGAAAACACAGTTATCAATACTAGTTAGAGAA110.17684887459807075No Hit
TGAGTAACCGCCACATAGTGAAAACACAGTTATCCATACTAGTTAGAGAA90.14469453376205788No Hit
TGAGTAACCGCAACATAGTGAAAACACAGTTATCCATACTAGGTAGAGAA80.12861736334405144No Hit
TGGTAACCGCAACATAGTGAAAACACAGTTATCCATACTAGTTAGAGAAC80.12861736334405144No Hit
TGAGTAACCGCAACATAGTGAAAACACAGTTAGCCATACTAGTGAGAGAA80.12861736334405144No Hit
TGAGTAACCGCAACATAGTGAAAACACAGTGATCCATACTAGTTAGAGAA70.11254019292604502No Hit
TGAGTAACCGCAACAGAGTGAAAACACAGTTATCCATACTAGTTAGAGAA70.11254019292604502No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)