FastQCFastQC Report
Mon 17 Apr 2023
000000000-KWF5G_l01_n02_ELP3B4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-KWF5G_l01_n02_ELP3B4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5724
Sequences flagged as poor quality0
Sequence length301
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGAGTAACCGCAACATAGTGAAAACACAGTTATCCATACTAGTTAGAGAA504388.10272536687631No Hit
TGCGTAACCGCAACATAGTGAAAACACAGTTATCCATACTAGTTAGAGAA981.7120894479385047No Hit
TGGGTAACCGCAACATAGTGAAAACACAGTTATCCATACTAGTTAGAGAA480.8385744234800839No Hit
TGAGTAACCGCAACATAGTGAAAACACAGTTATCCATACTAGTGAGAGAA290.506638714185884No Hit
TGAGTAACCGCAACATAGTGAAAACACAGTTATCCATACTCGTTAGAGAA150.2620545073375262No Hit
TGAGTAACCGCAACATAGTGAACACACAGTTATCCATACTAGTTAGAGAA140.2445842068483578No Hit
TGAGTAACCGCAACATAGTGAAAACACAGTTAGCCATACTAGTTAGAGAA120.20964360587002098No Hit
TGAGGAACCGCAACATAGTGAAAACACAGTTATCCATACTAGTTAGAGAA90.15723270440251574No Hit
TGAGTAACCGCAACATAGTGAAAACACAGTTATCAATACTAGTTAGAGAA90.15723270440251574No Hit
TGAGTAACCGCAACATAGTGAAAACACAGTTATCTATACTAGTTAGAGAA80.1397624039133473No Hit
TGAGTAACCGCAACATAGTGAAAACACAGTTATCCATACGAGTTAGAGAA80.1397624039133473No Hit
TGAGGAACCGCAACATAGTGAAAACACAGTTATCCATACTAGTGAGAGAA70.1222921034241789No Hit
TGAGTAACCGCCACATAGTGAAAACACAGTTATCCATACTAGTTAGAGAA70.1222921034241789No Hit
TGGTAACCGCAACATAGTGAAAACACAGTTATCCATACTAGTTAGAGAAC70.1222921034241789No Hit
TGAGTAACCGCAACAGAGTGAAAACACAGTTATCCATACTAGTTAGAGAA60.10482180293501049No Hit
TGAGTAACCGCAACATAGTGAAAACACAGTTAGCCATACTAGTGAGAGAA60.10482180293501049No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)