FastQCFastQC Report
Mon 17 Apr 2023
000000000-KWF5G_l01_n02_ELP3B12.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-KWF5G_l01_n02_ELP3B12.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5850
Sequences flagged as poor quality0
Sequence length301
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGAGTAACCGCAACATAGTGAAAACACAGTTATCCATACTAGTTAGAGAA511687.45299145299145No Hit
TGCGTAACCGCAACATAGTGAAAACACAGTTATCCATACTAGTTAGAGAA1141.948717948717949No Hit
TGGGTAACCGCAACATAGTGAAAACACAGTTATCCATACTAGTTAGAGAA510.8717948717948718No Hit
TGAGTAACCGCAACATAGTGAAAACACAGTTATCCATACTAGTGAGAGAA230.3931623931623932No Hit
TGAGTAACCGCAACATAGTGAAAACACAGTTAGCCATACTAGTTAGAGAA180.3076923076923077No Hit
TGGTAACCGCAACATAGTGAAAACACAGTTATCCATACTAGTTAGAGAAC120.20512820512820512No Hit
TGAGTAACCGCAACATAGTGAACACACAGTTATCCATACTAGTTAGAGAA100.17094017094017094No Hit
TGAGTAACCGCAACATAGTGAAAACACAGTTATCCATACTCGTTAGAGAA100.17094017094017094No Hit
TGAGTAACCGCAACATAGTGAAAACACAGTTATCAATACTAGTTAGAGAA100.17094017094017094No Hit
TGAGTAACCGCAACATAGTGAAAACACAGTTAGCCATACTAGTGAGAGAA90.15384615384615385No Hit
TGAGTAACCGCAACATAGTGAAAACACAGTTATCCATACGAGTTAGAGAA80.13675213675213677No Hit
TGAGTAACCGCAACATAGTGAAAACACAGTTATCCATACGAGTGAGAGAA70.11965811965811966No Hit
TGAGTAACCGCACCATAGTGAAAACACAGTTATCCATACTAGTTAGAGAA70.11965811965811966No Hit
TGAGTAACCGCAACATAGTGAAAACACAGTTATCCATACTAGTTAGAGCA70.11965811965811966No Hit
TGAGTAACCGCCACATAGTGAAAACACAGTTATCCATACTAGTTAGAGAA70.11965811965811966No Hit
TGAGTAACCGCACATAGTGAAAACACAGTTATCCATACTAGTTAGAGAAC60.10256410256410256No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)