FastQCFastQC Report
Mon 17 Apr 2023
000000000-KWF5G_l01_n02_ELP3A6.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-KWF5G_l01_n02_ELP3A6.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5326
Sequences flagged as poor quality0
Sequence length301
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGAGTAACCGCAACATAGTGAAAACACAGTTATCCATACTAGTTAGAGAA491592.28313931656027No Hit
TGCGTAACCGCAACATAGTGAAAACACAGTTATCCATACTAGTTAGAGAA1362.5535110777318812No Hit
TGAGTAACCGCAACATAGTGAACACACAGTTATCCATACTAGTTAGAGAA170.31918888471648516No Hit
TGAGTAACCGCAACATAGTGAAAACACAGTTATCAATACTAGTTAGAGAA160.3004130679684566No Hit
TGAGTAACCGCAACATAGTGAAAACACAGTTATCCATACTCGTTAGAGAA130.244085617724371No Hit
TGAGTAACCGCAACATAGTGCAAACACAGTTATCCATACTAGTTAGAGAA120.22530980097634246No Hit
AAGGCCACGTCATCCCCACCTTCCTCCGGTTTGTCACCGGCAGTCTCCTT90.16898235073225684No Hit
TGAGTAACCGCCACATAGTGAAAACACAGTTATCCATACTAGTTAGAGAA80.1502065339842283No Hit
TGAGTAACCGCAACATAGTGAAAACACAGTTATCCATACTAGTTAGAGCA70.13143071723619978No Hit
TGAGTAACCGCAACATAGTGACAACACAGTTATCCATACTAGTTAGAGAA70.13143071723619978No Hit
TGAGTCACCGCAACATAGTGAAAACACAGTTATCCATACTAGTTAGAGAA70.13143071723619978No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)