FastQCFastQC Report
Mon 17 Apr 2023
000000000-KWF5G_l01_n02_ELP2H7.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-KWF5G_l01_n02_ELP2H7.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2484
Sequences flagged as poor quality0
Sequence length301
%GC53

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGGCCACGTCATCCCCACCTTCCTCCGGTTTGTCACCGGCAGTCACCTT176070.85346215780999No Hit
AAGGCCACGTCATCCCCACCTTCCTCCGGTTTGTCACCGGCAGTCTCCTT1897.608695652173914No Hit
AAGGCCACGTCATCCCCTCCTTCCTCCGGTTTGTCACCGGCAGTCACCTT431.7310789049919486No Hit
AAAGCCACGTCATCCCCACCTTCCTCCGGTTTGTCACCGGCAGTCACCTT421.6908212560386473No Hit
AAGGCCACGTCATCCCCACCTTCCTCCGGTTTGTCACCGTCAGTCACCTT371.4895330112721417No Hit
AAGGCCACGTCATCCCCACCTTCCTCCGGTTTGTCACCGGAAGTCACCTT200.8051529790660225No Hit
AAGGCCACGTCCTCCCCACCTTCCTCCGGTTTGTCACCGGCAGTCACCTT130.5233494363929146No Hit
AAGGCCACGTCATCCCCACCTTCCTCCGGTTTGTCACAGGCAGTCACCTT120.4830917874396135No Hit
AAGGCCACGTCATCCCCACCTTCCTCCGGTTTGTCACCGGCATTCACCTT100.40257648953301123No Hit
AAGGCCACGTCATCCCACCTTCCTCCGGTTTGTCACCGGCAGTCACCTTA70.28180354267310787No Hit
AAGGCCCCGTCATCCCCACCTTCCTCCGGTTTGTCACCGGCAGTCACCTT60.24154589371980675No Hit
AAAGCCACGTCATCCCCACCTTCCTCCGGTTTGTCACCGGCAGTCTCCTT60.24154589371980675No Hit
AAGGCCACGTCATCACCACCTTCCTCCGGTTTGTCACCGGCAGTCACCTT50.20128824476650561No Hit
AAGGCCACGTCATCCCCACCTTCCTCCGGTTTGTCATCGGCAGTCACCTT50.20128824476650561No Hit
AAGGCCACGTCATCCCCACCTTCCTCCGGTTTTTCACCGGCAGTCACCTT50.20128824476650561No Hit
AAGGCCACGTCATCCTCACCTTCCTCCGGTTTGTCACCGGCAGTCACCTT50.20128824476650561No Hit
AAGGCCACGTCATCCCCTCCTTCCTCCGGTTTGTCACCGGCAGTCTCCTT50.20128824476650561No Hit
AAGGCCACGTCATCCCCACCTTCCTCCGGTTTGTCACCGGCAGACACCTT50.20128824476650561No Hit
AAGGCCACGTCATCCCCACCTTCCTACGGTTTGTCACCGGCAGTCACCTT50.20128824476650561No Hit
AAGGCCACGTCATCCCCACCTTCCTCCGGTTTGTCACCGTCAGTCTCCTT40.1610305958132045No Hit
ACGGCCACGTCATCCCCACCTTCCTCCGGTTTGTCACCGGCAGTCACCTT40.1610305958132045No Hit
AAGGCCACGTCATCCCCACCTTCCTCCGGTTTGTCACCGACAGTCACCTT40.1610305958132045No Hit
AAGGCCACGTCATCCCCACCTTCCTCCGGTTTGTCACCGGCAGTCCCCTT40.1610305958132045No Hit
AAGGCCACGTCATCCCCACCTTCCTCCGGTTTGTAACCGGCAGTCACCTT40.1610305958132045No Hit
AAGGCCACGTCATCCCCACCTTCCTCCGGTTTGTCACCGGCAGTAACCTT40.1610305958132045No Hit
AAGGCCACGTCATCCCCACCTTCCTCCGGTTTGTCACCGGAAGTCTCCTT30.12077294685990338No Hit
AAGGCCACGTCATCCCCACCTTCCTCCGGTTTGTCACCGGCAGCCACCTT30.12077294685990338No Hit
AAGGCCCGTCATCCCCACCTTCCTCCGGTTTGTCACCGGCAGTCACCTTA30.12077294685990338No Hit
AAGGCCACGTCATCCCCACCTTCCTCCGGTTTGTCACCGGCAGTCACCAT30.12077294685990338No Hit
AAGGCCACGTCATCCCCACCTTCATCCGGTTTGTCACCGGCAGTCACCTT30.12077294685990338No Hit
AAGGCCACGTCATCCCCACCTTCCTCCGGTTTGTCACAGGAAGTCACCTT30.12077294685990338No Hit
AAGGCCACGTCATCCCCACCTTCCTCCGGTTTGTCACCCGCAGTCACCTT30.12077294685990338No Hit
AAGGCCACGTCATCCCCACCTACCTCCGGTTTGTCACCGGCAGTCACCTT30.12077294685990338No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)