FastQCFastQC Report
Mon 17 Apr 2023
000000000-KWF5G_l01_n02_ELP2G7.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-KWF5G_l01_n02_ELP2G7.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences58
Sequences flagged as poor quality0
Sequence length301
%GC52

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGGCCACGTCATCCCCACCTTCCTCCGGTTTGTCACCGGCAGTCTCCTT4374.13793103448276No Hit
AAAGCCACGTCATCCCCACCTTCCTCCGGTTTGTCACCGGCAGTCTCCTT46.896551724137931No Hit
AAGGCCACGTATCCCCACCTTCCTCCGGTTTGTCACCGGCAGTCTCCTTA23.4482758620689653No Hit
AAGGCCACGTCATCCCCTCTTTCCTCCGGTTTGTCACCGGCAGTCTCCTT23.4482758620689653No Hit
AAGGGCCACGTCATCCCCACCTTCCTCCGGTTTGTCACCGGCAGTCTCCT23.4482758620689653No Hit
AAGGCCACGTCATCCCCACCTTCCTCCGGTTTGTCACCGGCAGTCACCTT23.4482758620689653No Hit
AAGGCCACGTCATCCCCACCTTCCTCCGGTTTGTCACAGGCAGTCTCCTT11.7241379310344827No Hit
AAGGCAACGTCAACCTCACCTACCTCCGGTTTTTCACCCGCATTATACTT11.7241379310344827No Hit
AAGGCCACGTCATCCCCACCTTCCTCTCGGCTTATCACCTGCAGTCCCCT11.7241379310344827No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)