FastQCFastQC Report
Mon 17 Apr 2023
000000000-KWF5G_l01_n02_ELP2G4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-KWF5G_l01_n02_ELP2G4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2755
Sequences flagged as poor quality0
Sequence length301
%GC53

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGGCCACGTCATCCCCACCTTCCTCCGGTTTGTCACCGGCAGTCACCTT195170.81669691470054No Hit
AAGGCCACGTCATCCCCACCTTCCTCCGGTTTGTCACCGGCAGTCTCCTT1926.969147005444647No Hit
AAGGCCACGTCATCCCCACCTTCCTCCGGTTTGTCACCGGCAGTCTCTCT562.0326678765880217No Hit
AAGGCCACGTCATCCCCTCCTTCCTCCGGTTTGTCACCGGCAGTCACCTT541.9600725952813067No Hit
AAAGCCACGTCATCCCCACCTTCCTCCGGTTTGTCACCGGCAGTCACCTT461.6696914700544463No Hit
AAGGCCACGTCATCCCCACCTTCCTCCGGTTTGTCACCGTCAGTCACCTT281.0163339382940109No Hit
AAGGCCACGTCATCCCCACCTTCCTCCGGTTTGTCACCGGAAGTCACCTT240.8711433756805809No Hit
AAGGCCACGTCATCCCCACCTTCCTCTCGGCTTATCACCGGCAGTCCCCT200.7259528130671506No Hit
AAGGCCACGTCATCCCCACCTTCCTACGGTTTGTCACCGGCAGTCACCTT110.3992740471869328No Hit
AAAGCCACGTCATCCCCACCTTCCTCCGGTTTGTCACCGGCAGTCTCCTT80.2903811252268602No Hit
AAGGCCACGTCATCCTCACCTTCCTCCGGTTTGTCACCGGCAGTCACCTT70.2540834845735027No Hit
AAGGCCACGTCATCCCACCTTCCTCCGGTTTGTCACCGGCAGTCACCTTA60.21778584392014522No Hit
AAGGCCACTCATCCCCACCTTCCTCCGGTTTGTCACCGGCAGTCACCTTA60.21778584392014522No Hit
AAGGCCACGTCAGCCCCACCGGCCGCCGGTTGGGCACCGGCAGTCACCGG60.21778584392014522No Hit
AAGGCCACGTCATCCCCACCTTCCTCCGGTTTGTCACAGGCAGTCACCTT50.18148820326678766No Hit
AAGGCCACGGCATCCCCACCTTCCTCCGGTTTGTCACCGGCAGTCACCTT50.18148820326678766No Hit
AAGGCAACGTCATCCCCACCTTCCTCCGGTTTGTCACCGGCAGTCACCTT50.18148820326678766No Hit
AAGGCCACGTCATCCCCACCTTCCTCCGGTTTGTCACCGGCAGTAACCTT50.18148820326678766No Hit
AAGGCCACGTCATCACCACCTTCCTCCGGTTTGTCACCGGCAGTCACCTT40.1451905626134301No Hit
AAGGCCACGTCATCCCCACCTTCCTCCGGTTTGTCACCGGCAGTCACCAT40.1451905626134301No Hit
AAGGCCACGTCATCCCCACCTTCCTCCGGTTTGTCACCGGCAGTCACCGT40.1451905626134301No Hit
AAGGCCACGTCCTCCCCACCTTCCTCCGGTTTGTCACCGGCAGTCACCTT40.1451905626134301No Hit
AAGGCCACGTCATCCCCACCTTCCTCCGGTTTGTCACCGTCATTCACCTT40.1451905626134301No Hit
AAGGCCACGTCATCCCCACCTTCCTCCGGTTTGTCATCGGCAGTCACCTT30.10889292196007261No Hit
AAGGCCACGTCATCCCCTCCTTCCTCCGGTTTGTCACCGGCAGTCTCCTT30.10889292196007261No Hit
AAGGCCACGTCATCCCCACCTTCCTCCGGTTTGTCACCGGCATTCACCTT30.10889292196007261No Hit
AAGGCCACGTCATCCCCACCTTCCTCCGGTTTTTCACCGGCATTCACCTT30.10889292196007261No Hit
AAGGCCACGTCATCCCCACCTTCCTCCGGTTTGTCACCGGCGGTCACCTT30.10889292196007261No Hit
AAGGCCACGTCATCCCCACCTTCCTCCGGTTTGTCACCGGCAGTCAACTT30.10889292196007261No Hit
AAGGCCACGTCATCCCCACCTTCATCCGGTTTGTCACCGGCAGTCTCCTT30.10889292196007261No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)