FastQCFastQC Report
Mon 17 Apr 2023
000000000-KWF5G_l01_n02_ELP2B8.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-KWF5G_l01_n02_ELP2B8.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5206
Sequences flagged as poor quality0
Sequence length301
%GC52

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGGCCACGTCATCCCCACCTTCCTCCGGTTTGTCACCGGCAGTCTCCTT398676.56550134460238No Hit
AAAGCCACGTCATCCCCACCTTCCTCCGGTTTGTCACCGGCAGTCTCCTT1222.3434498655397618No Hit
AAGGCCACGTCATCCCCACCTTCCTCCGGTTTGTCACCGTCAGTCTCCTT841.6135228582404917No Hit
AAGGCCACGTCATCCCCTCCTTCCTCCGGTTTGTCACCGGCAGTCTCCTT831.5943142527852476No Hit
AAGGCCACGTCATCCCCACCTTCCTCCGGTTTGTCACCGGAAGTCTCCTT420.8067614291202458No Hit
AAGGCCACGTCATCCCCACCTTCCTCCGGTTTGTCACAGGCAGTCTCCTT290.5570495582020745No Hit
AAGGCCACGTCATCCCCACCTTCCTCCGGTTTGTCACCGGCATTCTCCTT220.42258932001536686No Hit
AAGGCCACGTCATCACCACCTTCCTCCGGTTTGTCACCGGCAGTCTCCTT180.3457548981943911No Hit
AAGGCCACGTCATCCTCACCTTCCTCCGGTTTGTCACCGGCAGTCTCCTT180.3457548981943911No Hit
AAGGCCACGTCATCCCCACCTTCCTCCGGTTTTTCACCGGCAGTCTCCTT160.3073376872839032No Hit
AAGGCCACGTCATCCCCACCTTCCTCCGGTTTGTCACCGGCAGTCTCTCT160.3073376872839032No Hit
AAGGCCACGTCATCCCCACCTTCCTCCGGTTTGTCACCCGCAGTCTCCTT160.3073376872839032No Hit
AAGGCCACGTCCTCCCCACCTTCCTCCGGTTTGTCACCGGCAGTCTCCTT130.24971187091817135No Hit
AAGGCAACGTCATCCCCACCTTCCTCCGGTTTGTCACCGGCAGTCTCCTT120.23050326546292738No Hit
AAGGCCACGTCATCCCCACCTTCCTACGGTTTGTCACCGGCAGTCTCCTT110.21129466000768343No Hit
AAGGCCACGTCATCCCCACCTTCCTCCGGTTTGTCATCGGCAGTCTCCTT100.1920860545524395No Hit
AAGGCCACGTCATCCCACCTTCCTCCGGTTTGTCACCGGCAGTCTCCTTA100.1920860545524395No Hit
AAGGCCACGTCATCCCCACCTTCCTCCGGTTTGTCACCGGCAGTATCCTT90.17287744909719555No Hit
AAGGCCACGTCATCCCCACCTTCCTCCGGTTTGTCACCGGCAGTCTACTT90.17287744909719555No Hit
AAGGCCACGTCATCCCCACCTTCCTCCGGTTTTTCACCGTCAGTCTCCTT80.1536688436419516No Hit
AAGGCCACGTCATCCCCACCTTCCTCCGGTTTGTCACCGTAAGTCTCCTT80.1536688436419516No Hit
AAGGCCACGTCATCCCCACCTTCCTCCGGTTTGTCACCGGCAGTCACCTT80.1536688436419516No Hit
AAGGCCACGTCATCCCCACCTTCCTCCGGTTTTTCACCGGCATTCTCCTT70.13446023818670766No Hit
AAGGCCACGTCATCCCCACCTTCCTCCGGTTTGTCACCGGCAGTCTCCAT70.13446023818670766No Hit
AAGGCCACGTCATCCCCACCTTCCTCCGGTTTGTCACCGGCAGTCGCCTT70.13446023818670766No Hit
AAGGCCACGTCATCCCCACCTTCATCCGGTTTGTCACCGGCAGTCTCCTT70.13446023818670766No Hit
AAGGCCACGTCATCCCCACCTTCCTCCGGTTTGTCACCGTCATTCTCCTT60.11525163273146369No Hit
AAGGCACGTCATCCCCACCTTCCTCCGGTTTGTCACCGGCAGTCTCCTTA60.11525163273146369No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)