FastQCFastQC Report
Mon 17 Apr 2023
000000000-KWF5G_l01_n02_ELP2B4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-KWF5G_l01_n02_ELP2B4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9179
Sequences flagged as poor quality0
Sequence length301
%GC53

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGGCCACGTCATCCCCACCTTCCTCCGGTTTGTCACCGGCAGTCACCTT600265.38838653448089No Hit
AAGGCCACGTCATCCCCACCTTCCTCCGGTTTGTCACCGGCAGTCTCCTT5175.6324218324436215No Hit
AAGGCCACGTCATCCCCACCTTCCTCCGGTTTGTCACCGGCAGTCTCTCT1751.9065257653339145No Hit
AAAGCCACGTCATCCCCACCTTCCTCCGGTTTGTCACCGGCAGTCACCTT1731.884736899444384No Hit
AAGGCCACGTCATCCCCTCCTTCCTCCGGTTTGTCACCGGCAGTCACCTT1381.503431746377601No Hit
AAGGCCACGTCATCCCCACCTTCCTCCGGTTTGTCACCGTCAGTCACCTT1071.165704325089879No Hit
AAGGCCACGTCATCCCCACCTTCCTCTCGGCTTATCACCGGCAGTCCCCT971.056759995642227No Hit
AAGGCCACGTCATCCCCACCTTCCTCCGGTTTGTCACCGGAAGTCACCTT900.9804989650288704No Hit
AAGGCAACGTCATCCCCACCTTCCTCCGGTTTGTCACCGGCAGTCACCTT400.43577731779060896No Hit
AAGGCCACGTCATCACCACCTTCCTCCGGTTTGTCACCGGCAGTCACCTT270.29414968950866105No Hit
AAGGCCACGTCATCCCCACCTTCCTACGGTTTGTCACCGGCAGTCACCTT260.28325525656389583No Hit
AAGGCCACGTCATCCCCACCTTCCTCCGGTTTGTCACCGGCATTCACCTT240.2614663906743654No Hit
AAGGCCACGTCATCCTCACCTTCCTCCGGTTTGTCACCGGCAGTCACCTT210.22878309184006973No Hit
AAGGCCACGTCATCCCCACCTTCCTCCGGTTTGTCACCGGCAGTCCCCTT200.21788865889530448No Hit
AAGGCCACGTCATCCCCACCTTCATCCGGTTTGTCACCGGCAGTCACCTT190.2069942259505393No Hit
AAGGCCACGTCATCCCCACCTTCCTCCGGTTTGTCACAGGCAGTCACCTT190.2069942259505393No Hit
AAGGCCACGTCATCCCACCTTCCTCCGGTTTGTCACCGGCAGTCACCTTA180.19609979300577404No Hit
AAGGCCACGTCTCCCCACCTTCCTCCGGTTTGTCACCGGCAGTCACCTTA160.1743109271162436No Hit
AAGGCCACGTCATCCCCACCTTCCTCCGGTTTTTCACCGGCAGTCACCTT160.1743109271162436No Hit
AAGGCCACGTCATCCCCACCTTCCTCCGGTTTGTCACCGGCAGTAACCTT150.16341649417147835No Hit
AAAGCCACGTCATCCCCACCTTCCTCCGGTTTGTCACCGGCAGTCTCCTT150.16341649417147835No Hit
AAGGCCACGTCATCCCCACCTTCCTCCGGTTTGTCATCGGCAGTCACCTT140.15252206122671313No Hit
AAGGCCACGTCATCCCCACCTTCCTCCGGTTTGTCACCCGCAGTCACCTT140.15252206122671313No Hit
AAGGCCACGGCATCCCCACCTTCCTCCGGTTTGTCACCGGCAGTCACCTT140.15252206122671313No Hit
AAGGCCACGTCATCCCCACCTTCCTCCGGTTTGTCACCGGCGGTCTCCTT130.14162762828194791No Hit
AAGGCCACGTCATCCCCACCTTCCTCCGGTTTGTCACCGGCAGTCACCGT130.14162762828194791No Hit
AAGGCCACGTCATCCCCACCTTCCTCCGGTTTGTCACCGGCAGTCACCAT120.1307331953371827No Hit
AAGGCCACGTCCTCCCCACCTTCCTCCGGTTTGTCACCGGCAGTCACCTT110.11983876239241746No Hit
AAGGCCACGTCATCCCCACCTTCCTCCGGTTTGTCACCGTCAGTCTCCTT100.10894432944765224No Hit
AGGCCACGTCATCCCCACCTTCCTCCGGTTTGTCACCGGCAGTCACCTTA100.10894432944765224No Hit
AAGGCCACGTCATCCCCACCTTCCTCCGGTTTTTCACCGTCAGTCACCTT100.10894432944765224No Hit
AAGGCCACGTCATCCCCACCTTCCTCCGGTTTGTAACCGGCAGTCACCTT100.10894432944765224No Hit
AAGGCCACGTCATCCCCACCTTACTCCGGTTTGTCACCGGCAGTCACCTT100.10894432944765224No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)