FastQCFastQC Report
Mon 17 Apr 2023
000000000-KWF5G_l01_n02_ELP1G8.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-KWF5G_l01_n02_ELP1G8.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences52522
Sequences flagged as poor quality0
Sequence length301
%GC53

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGGCCACGTCATCCCCACCTTCCTCCGGTTTGTCACCGGCAGTCACCTT3520167.02143863523857No Hit
AAGGCCACGTCATCCCCACCTTCCTCCGGTTTGTCACCGGCAGTCTCCTT34416.551540306919006No Hit
AAGGCCACGTCATCCCCACCTTCCTCCGGTTTGTCACCGGCAGTCTCTCT12972.4694413769468033No Hit
AAAGCCACGTCATCCCCACCTTCCTCCGGTTTGTCACCGGCAGTCACCTT10632.0239137885076732No Hit
AAGGCCACGTCATCCCCTCCTTCCTCCGGTTTGTCACCGGCAGTCACCTT7981.5193633144206238No Hit
AAGGCCACGTCATCCCCACCTTCCTCTCGGCTTATCACCGGCAGTCCCCT7111.353718441795819No Hit
AAGGCCACGTCATCCCCACCTTCCTCCGGTTTGTCACCGTCAGTCACCTT5521.0509881573435893No Hit
AAGGCCACGTCATCCCCACCTTCCTCCGGTTTGTCACCGGAAGTCACCTT4420.841552111496135No Hit
AAGGCCACGTCATCCCCACCTTCCTCCGGTTTGTCACAGGCAGTCACCTT1730.329385781196451No Hit
AAGGCCACGTCATCCCACCTTCCTCCGGTTTGTCACCGGCAGTCACCTTA1350.2570351471764213No Hit
AAGGCCACGTCATCCCCACCTTCCTCCGGTTTGTCACCGGCATTCACCTT1270.2418034347511519No Hit
AAGGCCCCGTCATCCCCACCTTCCTCCGGTTTGTCACCGGCAGTCACCTT1190.22657172232588252No Hit
AAGGCCACGTCATCACCACCTTCCTCCGGTTTGTCACCGGCAGTCACCTT1090.20753208179429572No Hit
AAGGCCACGTCATCCCCACCTTCCTACGGTTTGTCACCGGCAGTCACCTT1080.20562811774113704No Hit
AAGGCCACGTCATCCCCACCTTCCTCCGGTTTGTCACCGGCAGTCCCCTT1030.19610829747534367No Hit
AAAGCCACGTCATCCCCACCTTCCTCCGGTTTGTCACCGGCAGTCTCCTT980.1865884772095503No Hit
AAGGCCACGTCATCCCCTCCTTCCTCCGGTTTGTCACCGGCAGTCTCCTT940.17897262099691558No Hit
AAGGCCACGTCCTCCCCACCTTCCTCCGGTTTGTCACCGGCAGTCACCTT940.17897262099691558No Hit
AAGGCCACGTCATCCCCACCTTCCTCCGGTTTGTCATCGGCAGTCACCTT880.16754883667796353No Hit
AAGGCCACGTCATCCCCACCTTCCTCCGGTTTGTCACCCGCAGTCACCTT860.16374090857164617No Hit
AAGGCCACGTCATCCCCACCTTCCTCCGGTTTGTCACCGGCGGTCTCCTT830.15802901641217013No Hit
AAGGCCACGTCATCCCCACCTTCCTCCGGCTTATCACCGGCAGTTTCCTT800.1523171242526941No Hit
AAGGCCACGTCATCCTCACCTTCCTCCGGTTTGTCACCGGCAGTCACCTT780.14850919614637675No Hit
AAGGCCACGTCATCCCCACCTTCCTCCGGTTTTTCACCGGCAGTCACCTT760.1447012680400594No Hit
AAGGCCACGTCATCCCCACCTTCCTCCGGTTTGTCACCGGCAGTCACCGT740.14089333993374206No Hit
AAGGCCACGGCATCCCCACCTTCCTCCGGTTTGTCACCGGCAGTCACCTT660.12566162750847265No Hit
AAGGCCACGTCATCCCCACCTTCCTCCGGTTTGTCACCGTCAGTCTCCTT660.12566162750847265No Hit
AAGGCACGTCATCCCCACCTTCCTCCGGTTTGTCACCGGCAGTCACCTTA580.11042991508320321No Hit
AAGGCCACGTCATCCCCACCTTCATCCGGTTTGTCACCGGCAGTCACCTT550.1047180229237272No Hit
AAGGCCACGTCATCCCCACCTTCCTCCGGTTTGTCACCGGCAGTCACCAT530.10091009481740984No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)