FastQCFastQC Report
Mon 17 Apr 2023
000000000-KWF5G_l01_n02_ELP1G6.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-KWF5G_l01_n02_ELP1G6.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2585
Sequences flagged as poor quality0
Sequence length301
%GC54

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGGCCACGTCATCCCCACCTTCCTCCGGTTTGTCACCGGCAGTCTCCTT206880.0No Hit
AAAGCCACGTCATCCCCACCTTCCTCCGGTTTGTCACCGGCAGTCTCCTT622.398452611218569No Hit
AAGGCCACGTCATCCCCTCCTTCCTCCGGTTTGTCACCGGCAGTCTCCTT411.586073500967118No Hit
AAGGCCACGTCATCCCCACCTTCCTCCGGTTTGTCACCGGAAGTCTCCTT341.3152804642166345No Hit
AAGGCCACGTCATCCCCACCTTCCTCCGGTTTGTCACCGTCAGTCTCCTT301.160541586073501No Hit
AAGGCCACGTCATCCCCACCTTCCTCCGGTTTGTCACCGGCAGTCTCTCT110.425531914893617No Hit
AAGGCCACGTCATCCCCACCTTCCTCCGGTTTGTCACCCGCAGTCTCCTT100.3868471953578337No Hit
AAGGCCACGTCCTCCCCACCTTCCTCCGGTTTGTCACCGGCAGTCTCCTT90.3481624758220503No Hit
AAGGCCACGTCATCCCCACCTTCCTCCGGTTTGTCACCGGCAGTCACCTT80.3094777562862669No Hit
AAGGCCACGTCATCACCACCTTCCTCCGGTTTGTCACCGGCAGTCTCCTT80.3094777562862669No Hit
AAGGCCACGTCATCCCCACCTTCCTCCGGTTTTTCACCGGCAGTCTCCTT80.3094777562862669No Hit
AGGCCACGTCATCCCCACCTTCCTCCGGTTTGTCACCGGCAGTCTCCTTA70.27079303675048355No Hit
AAGCCACGTCATCCCCACCTTCCTCCGGTTTGTCACCGGCAGTCTCCTTA70.27079303675048355No Hit
AAGGCCACGTCATCCCACCTTCCTCCGGTTTGTCACCGGCAGTCTCCTTA70.27079303675048355No Hit
AAGGCCACGTCATCCCCACCTTCCTACGGTTTGTCACCGGCAGTCTCCTT60.23210831721470018No Hit
AAGGCCACGTCATCCCCACCTTCCTCCGGTTTGTCACCGGCATTCTCCTT60.23210831721470018No Hit
AAGGCCACGTCTCCCCACCTTCCTCCGGTTTGTCACCGGCAGTCTCCTTA50.19342359767891684No Hit
AAGGCCACGTCATCCCCACCTTCCTCCGGTTTGTCACCGGCAGTATCCTT40.15473887814313345No Hit
AAGGCCCCGTCATCCCCACCTTCCTCCGGTTTGTCACCGGCAGTCTCCTT40.15473887814313345No Hit
AAGGCCACGTCATCCTCACCTTCCTCCGGTTTGTCACCGGCAGTCTCCTT40.15473887814313345No Hit
AAGGCCACGTCATCCCCACCTTCCTCCGGTTTGTCACCGGCAGTCTACTT40.15473887814313345No Hit
AAGGCCACGTCATCCCCACCTTCCTCCGGTTTGTCACCGTCATTCTCCTT40.15473887814313345No Hit
AAGGCCACGTCATCCCCACCTTCCTCCGGTTTGTCCCCGGCAGTCTCCTT40.15473887814313345No Hit
AAGGCCACGTCATCCCCACCTTCCTCCGGTTTGTCACCGGCAGTCGCCTT40.15473887814313345No Hit
AAGGCCACGTCATCCCCACCTTCCTCCGGTTTGTCACAGGCAGTCTCCTT40.15473887814313345No Hit
AAGGCCACGTCATCCCCACCTTCCTCCGGTTAGTCACCGGCAGTCTCCTT30.11605415860735009No Hit
AAGGCCACGTCATCCCCACCTTCCTCCGGTTTGACACCGGCAGTCTCCTT30.11605415860735009No Hit
AAGGCCACGTCATCCCCACCTTCCTCCGGTTTGTCATCGTCAGTCTCCTT30.11605415860735009No Hit
AAGGCCACGTCATCCCCACCTTCCTCCGGTTTGTAACCGGCAGTCTCCTT30.11605415860735009No Hit
AAAGCCACGTCATCCCCACCTTCCTCCGGTTTGTCACCGTCAGTCTCCTT30.11605415860735009No Hit
AAGGCCACGTCATCCCCACCTTCCTCCGGTTTGTCACCGGCAGTCTCCAT30.11605415860735009No Hit
AAAGGCCACGTCATCCCCACCTTCCTCCGGTTTGTCACCGGCAGTCTCCT30.11605415860735009No Hit
AAGGCCACGTCATCCCCACCTTCATCCGGTTTGTCACCGGCAGTCTCCTT30.11605415860735009No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)