FastQCFastQC Report
Mon 17 Apr 2023
000000000-KWF5G_l01_n02_ELP1D8.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-KWF5G_l01_n02_ELP1D8.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences44122
Sequences flagged as poor quality0
Sequence length301
%GC53

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGGCCACGTCATCCCCACCTTCCTCCGGTTTGTCACCGGCAGTCTCCTT1993845.188341416980194No Hit
AAGGCCACGTCATCCCCACCTTCCTCCGGTTTGTCACCGGCAGTCACCTT1415432.07923484882825No Hit
AAGGCCACGTCATCCCCACCTTCCTCCGGTTTGTCACCGGCAGTCTCTCT7831.7746249036761708No Hit
AAAGCCACGTCATCCCCACCTTCCTCCGGTTTGTCACCGGCAGTCTCCTT5481.2420107882688907No Hit
AAGGCCACGTCATCCCCTCCTTCCTCCGGTTTGTCACCGGCAGTCTCCTT5171.1717510538960156No Hit
AAAGCCACGTCATCCCCACCTTCCTCCGGTTTGTCACCGGCAGTCACCTT4210.954172521644531No Hit
AAGGCCACGTCATCCCCACCTTCCTCTCGGCTTATCACCGGCAGTCCCCT3390.7683241920130547No Hit
AAGGCCACGTCATCCCCTCCTTCCTCCGGTTTGTCACCGGCAGTCACCTT3380.7660577489687684No Hit
AAGGCCACGTCATCCCCACCTTCCTCCGGTTTGTCACCGTCAGTCTCCTT2860.648202710665881No Hit
AAGGCCACGTCATCCCCACCTTCCTCCGGTTTGTCACCGGAAGTCTCCTT2550.5779429762930057No Hit
AAGGCCACGTCATCCCCACCTTCCTCCGGTTTGTCACCGGAAGTCACCTT1870.4238248492815375No Hit
AAGGCCACGTCATCCCCACCTTCCTCCGGTTTGTCACCGTCAGTCACCTT1570.35583155795294863No Hit
AAGGCCACGTCATCCCCACCTTCCTCCGGCTTATCACCGGCAGTTTCCTT1130.2561080640043516No Hit
AAGGCCACGTCATCCCCACCTTCCTCCGGTTTGTCACCGGCATTCTCCTT900.20397987398576675No Hit
AAGGCCCCGTCATCCCCACCTTCCTCCGGTTTGTCACCGGCAGTCTCCTT810.18358188658719005No Hit
AAGGCCACGTCATCCCCACCTTCCTCCGGTTTGTCACAGGCAGTCTCCTT780.1767825574543312No Hit
AAGGCCACGTCATCCCACCTTCCTCCGGTTTGTCACCGGCAGTCTCCTTA760.17224967136575858No Hit
AAGGCCACGTCCTCCCCACCTTCCTCCGGTTTGTCACCGGCAGTCTCCTT680.1541181270114682No Hit
AAGGCCACGTCCTCCCCACCTTCCTCCGGTTTGTCACCGGCAGTCACCTT650.14731879787860933No Hit
AAGGCCACGTCATCACCACCTTCCTCCGGTTTGTCACCGGCAGTCTCCTT600.13598658265717783No Hit
AAGGCCACGTCATCCCCACCTTCCTCCGGTTTGTCACCGGCAGTCCCCTT600.13598658265717783No Hit
AAGGCCACGTCATCCCACCTTCCTCCGGTTTGTCACCGGCAGTCACCTTA570.12918725352431892No Hit
AAGGCCCCGTCATCCCCACCTTCCTCCGGTTTGTCACCGGCAGTCACCTT560.12692081048003265No Hit
AAGGCCACGTCATCCCCACCTTCCTACGGTTTGTCACCGGCAGTCTCCTT560.12692081048003265No Hit
AAGGCCACGTCATCCCCACCTTCCTCCGGTTTGTCACAGGCAGTCACCTT550.12465436743574634No Hit
AAGGCCACGTCATCCCCACCTTCCTCCGGTTTTTCACCGGCAGTCTCCTT500.11332215221431487No Hit
AAGGCCACGTCATCCCCACCTTCCTCCGGTTTGTCACCCGCAGTCTCCTT500.11332215221431487No Hit
AAGGCCACGTCATCCCCACCTTCCTCCGGTTTGTCACCGGCAGTCGCCTT490.11105570917002855No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)