FastQCFastQC Report
Mon 17 Apr 2023
000000000-KWF5G_l01_n02_ELP1C8.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-KWF5G_l01_n02_ELP1C8.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences61946
Sequences flagged as poor quality0
Sequence length301
%GC53

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGGCCACGTCATCCCCACCTTCCTCCGGTTTGTCACCGGCAGTCACCTT3745860.4687954024473No Hit
AAGGCCACGTCATCCCCACCTTCCTCCGGTTTGTCACCGGCAGTCTCCTT52028.397636651276919No Hit
AAGGCCACGTCATCCCCACCTTCCTCCGGTTTGTCACCGGCAGTCTCTCT15532.507022245181287No Hit
AAAGCCACGTCATCCCCACCTTCCTCCGGTTTGTCACCGGCAGTCACCTT11041.78219739773351No Hit
AAGGCCACGTCATCCCCTCCTTCCTCCGGTTTGTCACCGGCAGTCACCTT8391.3544054499079843No Hit
AAGGCCACGTCATCCCCACCTTCCTCCGGTTTGTCACCGTCAGTCACCTT7041.136473702902528No Hit
AAGGCCACGTCATCCCCACCTTCCTCTCGGCTTATCACCGGCAGTCCCCT6921.1171019920575986No Hit
AAGGCCACGTCATCCCCACCTTCCTCCGGTTTGTCACCGGAAGTCACCTT5890.9508281406386208No Hit
AAGGCCACGTCATCCCCACCTTCCTCCGGTTTGTCACCGGCGGTCTCCTT3790.6118232008523553No Hit
AAGGCCACGTCATCCCCACCTTCCTCCGGTTTGTCACAGGCAGTCACCTT2190.35353372291996255No Hit
AAAGCCACGTCATCCCCACCTTCCTCCGGTTTGTCACCGGCAGTCTCCTT1660.26797533335485746No Hit
AAGGCCACGTCATCCCCACCTTCCTCCGGTTTGTCACCGGCATTCACCTT1640.26474671488070256No Hit
AAGGCCACGTCATCCCCACCTTCCTCCGGCTTATCACCGGCAGTTTCCTT1600.2582894779323927No Hit
AAGGCCCCGTCATCCCCACCTTCCTCCGGTTTGTCACCGGCAGTCACCTT1490.24053207632454074No Hit
AAGGCCACGTCATCCCACCTTCCTCCGGTTTGTCACCGGCAGTCACCTTA1480.2389177670874633No Hit
AAGGCCACGTCATCACCACCTTCCTCCGGTTTGTCACCGGCAGTCACCTT1420.22923191166499854No Hit
AAGGCCACGTCATCCCCACCTTCCTACGGTTTGTCACCGGCAGTCACCTT1330.21470312853130147No Hit
AAGGCCACGTCCTCCCCACCTTCCTCCGGTTTGTCACCGGCAGTCACCTT1270.20501727310883672No Hit
AAGGCCACGTCATCCTCACCTTCCTCCGGTTTGTCACCGGCAGTCACCTT1250.2017886546346818No Hit
AAGGCCACGTCATCCCCTCCTTCCTCCGGTTTGTCACCGGCAGTCTCCTT1240.20017434539760437No Hit
AAGGCCACGTCATCCCCACCTTCCTCCGGTTTGTCATCGGCAGTCACCTT1220.19694572692344944No Hit
AAGGCCACGTCATCCCCACCTTCCTCCGGTTTTTCACCGGCAGTCACCTT1130.18241694378975237No Hit
AAGGCCACGTCATCCCCACCTTCCTCCGGTTTGTCACCCGCAGTCACCTT1120.1808026345526749No Hit
AAGGCCACGTCATCCCCACCTTCCTCCGGTTTGTCACCGTCAGTCTCCTT1050.16950246989313272No Hit
AAGGCCACGTCATCCCCACCTTCCTCCGGTTTGTCACCGGCAGTCCCCTT980.15820230523359055No Hit
AAGGCCACGTCATCCCCACCTTCCTCCGGTTTGTCACCGGAAGTCTCCTT840.13560197591450618No Hit
AAGGCCACGTCATCCCCACCTTCCTCCGGTTTGTCACCGGCAGTCACCGT820.13237335744035128No Hit
AAGGCCACGTCATCCCCACCTTCATCCGGTTTGTCACCGGCAGTCACCTT810.1307590482032738No Hit
AAGGCAACGTCATCCCCACCTTCCTCCGGTTTGTCACCGGCAGTCACCTT810.1307590482032738No Hit
AAGGCCACGGCATCCCCACCTTCCTCCGGTTTGTCACCGGCAGTCACCTT770.124301811254964No Hit
AAGGCCACGTCATCCCCACCTTCCTCCGGTTTGTCACCGGCAGTCACCAT710.11461595583249927No Hit
AAGGCCACGTCATCCCCACCTTCCTCCGGTTTGTCACCGGCAGTAACCTT690.11138733735834437No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)