FastQCFastQC Report
Mon 17 Apr 2023
000000000-KWF5G_l01_n02_ELP1C3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-KWF5G_l01_n02_ELP1C3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31654
Sequences flagged as poor quality0
Sequence length301
%GC53

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGGCCACGTCATCCCCACCTTCCTCCGGTTTGTCACCGGCAGTCTCCTT1279640.42459088898717No Hit
AAGGCCACGTCATCCCCACCTTCCTCCGGTTTGTCACCGGCAGTCACCTT954430.151007771529663No Hit
AAGGCCACGTCATCCCCACCTTCCTCCGGTTTGTCACCGGCAGTCTCTCT8802.780059392177924No Hit
AAGGCCACGTCATCCCCACCTTCCTCTCGGCTTATCACCGGCAGTCCCCT7802.4641435521577053No Hit
AAAGCCACGTCATCCCCACCTTCCTCCGGTTTGTCACCGGCAGTCTCCTT3951.2478675680798637No Hit
AAAGCCACGTCATCCCCACCTTCCTCCGGTTTGTCACCGGCAGTCACCTT3471.0962279648701585No Hit
AAGGCCACGTCATCCCCTCCTTCCTCCGGTTTGTCACCGGCAGTCTCCTT2830.8940418272572187No Hit
AAGGCCACGTCATCCCCACCTTCCTCCGGTTTGTCACCGTCAGTCTCCTT2330.7360839072471094No Hit
AAGGCCACGTCATCCCCACCTTCCTCCGAGTTGACCCCGGCAGTCTCCTA2310.729765590446705No Hit
AAGGCCACGTCATCCCCACCTTCCTCCGGCTTATCACCGGCAGTTTCCTT2070.6539457888418526No Hit
AAGGCCACGTCATCCCCTCCTTCCTCCGGTTTGTCACCGGCAGTCACCTT1750.5528527200353825No Hit
AAGGCCACGTCATCCCCACCTTCCTCCGGTTTGTCACCGGAAGTCTCCTT1520.4801920768307323No Hit
AAGGCCACGTCATCCCCACCTTCCTCCGGTTTGTCACCGTCAGTCACCTT1500.4738737600303279No Hit
AAGGCCACGTCATCCCCACCTTCCTCCGGTTTGTCACCGGAAGTCACCTT1370.4328047008276995No Hit
AAGGCCACGTCATCCCCACCTTCCTCCGGTTTGTCACCGGCGGTCTCCTT1140.36014405762304924No Hit
AAGGCCACGTCATCCCCACCTTCCTCCGGTTTGTCACAGGCAGTCTCCTT730.2306185632147596No Hit
AAGGCCACGTCATCCCACCTTCCTCCGGTTTGTCACCGGCAGTCTCCTTA720.2274594048145574No Hit
AAGGCCACGTCATCCCCACCTTCCTCCGGTTTGTCACCGGCAGTCCCCTT610.19270866241233334No Hit
AAGGCCACGTCATCCCCACCTTCCTCCGGTTTGTCACCGGCATTCTCCTT600.18954950401213116No Hit
AAGGCCACGTCATCCCCACCTTCCTCCGGTTTTTCACCGGCAGTCTCCTT520.16427623681051368No Hit
AAGGCCACGTCATCCTCACCTTCCTCCGGTTTGTCACCGGCAGTCTCCTT520.16427623681051368No Hit
AAGGCCACGTCATCACCACCTTCCTCCGGTTTGTCACCGGCAGTCTCCTT500.1579579200101093No Hit
AAGGCCACGTCATCCCCACCTTCCTCCGGTTTGTCACCCGCAGTCTCCTT470.14848044480950273No Hit
AAGGCCACGTCATCCCCACCTTCCTCCGGTTTGTCACCGGCATTCACCTT450.14216212800909836No Hit
AAGGCCACGTCATCCCCACCTTCCTACGGTTTGTCACCGGCAGTCTCCTT430.135843811208694No Hit
AAGGCCACGTCATCCCCACCTTCCTACGGTTTGTCACCGGCAGTCACCTT420.13268465280849182No Hit
AAGGCCACGTCATCCCCACCTTCCTCCGGTTTGTCACCGGCAGTCGCCTT410.12952549440828964No Hit
AAGGCCACGTCATCCCCACCTTCCTCCGGTTTGTCACAGGCAGTCACCTT400.12636633600808744No Hit
AAGGCCACGTCATCCCCACCTTCCTCCGGTTTGTCACCGGCAGTATCCTT360.1137297024072787No Hit
AAGGCCACGTCATCACCACCTTCCTCCGGTTTGTCACCGGCAGTCACCTT360.1137297024072787No Hit
AAGGCCACGTCATCCCCACCTTCATCCGGTTTGTCACCGGCAGTCTCCTT340.10741138560687433No Hit
AAGGCCACGTCATCCCCACCTTCCTCCGGTTTGTCACCGGCAGTCTACTT330.10425222720667215No Hit
AAGGCCACGTCATCCCCACCTTCCTCCGGTTTGTCATCGGCAGTCACCTT320.10109306880646995No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)