FastQCFastQC Report
Mon 17 Apr 2023
000000000-KWF5G_l01_n02_ELP1B5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-KWF5G_l01_n02_ELP1B5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences39279
Sequences flagged as poor quality0
Sequence length301
%GC53

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGGCCACGTCATCCCCACCTTCCTCCGGTTTGTCACCGGCAGTCTCCTT2852272.61386491509458No Hit
AAAGCCACGTCATCCCCACCTTCCTCCGGTTTGTCACCGGCAGTCTCCTT8862.2556582397718885No Hit
AAGGCCACGTCATCCCCTCCTTCCTCCGGTTTGTCACCGGCAGTCTCCTT6741.7159296316097659No Hit
AAGGCCACGTCATCCCCACCTTCCTCCGGTTTGTCACCGTCAGTCTCCTT5641.4358817688841365No Hit
AAGGCCACGTCATCCCCACCTTCCTCCGGTTTGTCACCGGAAGTCTCCTT4131.0514524300516814No Hit
AAGGCCACGTCATCCCCACCTTCCTCCGGTTTGTCACCGGCATTCTCCTT1630.41498001476616003No Hit
AAGGCCACGTCATCACCACCTTCCTCCGGTTTGTCACCGGCAGTCTCCTT1310.33351154560961327No Hit
AAGGCCACGTCATCCCCACCTTCCTCCGGTTTGTCACAGGCAGTCTCCTT1280.325873876626187No Hit
AAGGCCACGTCATCCCACCTTCCTCCGGTTTGTCACCGGCAGTCTCCTTA1180.30041498001476613No Hit
AAGGCCACGTCATCCTCACCTTCCTCCGGTTTGTCACCGGCAGTCTCCTT970.24695129713078234No Hit
AAGGCAACGTCATCCCCACCTTCCTCCGGTTTGTCACCGGCAGTCTCCTT940.23931362814735607No Hit
AAGGCCACGTCATCCCCACCTTCCTCCGGTTTGTCACCGGCAGTCACCTT920.2342218488250719No Hit
AAGGCCACGTCATCCCCACCTTCCTCCGGTTTGTCACCGGCAGTCGCCTT910.2316759591639298No Hit
AAGGCCACGTCATCCCCACCTTCCTCCGGTTTTTCACCGGCAGTCTCCTT910.2316759591639298No Hit
AAGGCCACGTCATCCCCACCTTCCTACGGTTTGTCACCGGCAGTCTCCTT910.2316759591639298No Hit
AAGGCCACGTCATCCCCACCTTCCTCCGGTTTGTCACCCGCAGTCTCCTT810.206217062552509No Hit
AAGGCCACGTCCTCCCCACCTTCCTCCGGTTTGTCACCGGCAGTCTCCTT710.18075816594108812No Hit
AAGGCCCCGTCATCCCCACCTTCCTCCGGTTTGTCACCGGCAGTCTCCTT670.17057460729651977No Hit
AAGGCCACGTCATCCCCACCTTCCTCCGGTTTGTCACCGTCATTCTCCTT620.15784515899080934No Hit
AAGGCCACGTCATCCCCACCTTCCTCCGGTTTGTCACCGGCAGTATCCTT600.15275337966852515No Hit
AAGGCCACGTCATCCCCACCTTCATCCGGTTTGTCACCGGCAGTCTCCTT590.15020749000738307No Hit
AAGGCCACGTCATCCCCACCTTCCTCCGGTTTGTCACCGGCAGTCTCCGT570.1451157106850989No Hit
AAGGCCACGTCATCCCCACCTTCCTCCGGTTTGTCACCGGCAGTCTACTT560.14256982102395682No Hit
CAGGCCACGTCATCCCCACCTTCCTCCGGTTTGTCACCGGCAGTCTCCTT530.13493215204053055No Hit
AAGGCCACGTCATCCCCACCTTCCTCCGGTTTGTCATCGGCAGTCTCCTT480.12220270373482012No Hit
AAGGCCACGTCATCCCCACCTTCCTCCGGTTTGTCACCGTAAGTCTCCTT480.12220270373482012No Hit
AAGGCCACGTCATCCCCACCTTCCTCCGGTTTGTCACCGGCAGTCTCTCT480.12220270373482012No Hit
AAGGCCACGTCATCCCCACCTTCCTCCGGTTTGTCACCGGCAGTCTCCAT440.11201914509025178No Hit
AAGGCCACGTCATCCCCACCTTCCTCCGGTTTTTCACCGTCAGTCTCCTT400.10183558644568345No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)