FastQCFastQC Report
Mon 17 Apr 2023
000000000-KWF5G_l01_n01_ELP4H8.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-KWF5G_l01_n01_ELP4H8.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3526
Sequences flagged as poor quality0
Sequence length301
%GC40

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATGAGTCAGCGTCGAACATGCAACATTTATTGCATGATACGTGGCATTT326392.54112308564946No Hit
GATGAGTCAGCGTCNAACATGCAACATTTATTGCATGATACGTGGCATTT982.7793533749290984No Hit
GATGAGTCAGCGTCGCACATGCAACATTTATTGCATGATACGTGGCATTT80.22688598979013047No Hit
ATGAGTCAGCGTCGAACATGCAACATTTATTGCATGATACGTGGCATTTC70.19852524106636413No Hit
GATGAGTCAGCGTCGAACATGCAACCTTTATTGCATGATACGTGGCATTT60.17016449234259784No Hit
GATGGTCAGCGTCGAACATGCAACATTTATTGCATGATACGTGGCATTTC50.14180374361883152No Hit
GATGAGTCAGCATCGAACATGCAACATTTATTGCATGATACGTGGCATTT50.14180374361883152No Hit
GATGAGTCAGCGTCGAACATGCAACATTTATTGCATGATACGTGGAATTT40.11344299489506524No Hit
GATGAGTCAGCGTCGAACATGCAACATTTATTGCATGCTACGTGGCATTT40.11344299489506524No Hit
GATGAGTAAGCGTCGAACATGCAACATTTATTGCATGATACGTGGCATTT40.11344299489506524No Hit
GATGAGTCAGCGTCGAACATGCAACATTTATTGCATGATACGTTGCATTT40.11344299489506524No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)