FastQCFastQC Report
Mon 17 Apr 2023
000000000-KWF5G_l01_n01_ELP4H7.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-KWF5G_l01_n01_ELP4H7.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3894
Sequences flagged as poor quality0
Sequence length301
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATGAGTCAGCGTCGAACATGCAACATTTATTGCATGATACGTGGCATTT361892.91217257318952No Hit
GATGAGTCAGCGTCNAACATGCAACATTTATTGCATGATACGTGGCATTT902.311248073959938No Hit
GATGAGTCAGCGTCGCACATGCAACATTTATTGCATGATACGTGGCATTT90.23112480739599386No Hit
GTGAGTCAGCGTCGAACATGCAACATTTATTGCATGATACGTGGCATTTC90.23112480739599386No Hit
GATGAGTCAGCGTCGAACATGCAACATTTATTGCATGATACGAGGCATTT70.17976373908577298No Hit
ATGAGTCAGCGTCGAACATGCAACATTTATTGCATGATACGTGGCATTTC60.15408320493066258No Hit
GATGAGTCAGCGTCGAACATGCAACATTTATTGCATGATACGTGGCCTTT50.12840267077555212No Hit
GATGAGTCAGCGTCGAACATGCACCATTTATTGCATGATACGTGGCATTT50.12840267077555212No Hit
GATGAGACAGCGTCGAACATGCAACATTTATTGCATGATACGTGGCATTT50.12840267077555212No Hit
GATGAGTCAGCGTCGAACATGCAACATTTCTTGCATGATACGTGGCATTT40.1027221366204417No Hit
GATGAGTCAGCGTCGAACATGCAACATTTATTGCATGATCCGTGGCATTT40.1027221366204417No Hit
GATGAGTCAGCGTCGAACATGCAACATTTATTGCATGATACGTGACATTT40.1027221366204417No Hit
GATGCGTCAGCGTCGAACATGCAACATTTATTGCATGATACGTGGCATTT40.1027221366204417No Hit
GATGAGTCAGCGTCGAACATGCAACATTTATTGCATGATAAGTGGCATTT40.1027221366204417No Hit
GATGAGTCAGCGTCGAACATGCAACATTTATTGCATGATACGTGGAATTT40.1027221366204417No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)