FastQCFastQC Report
Mon 17 Apr 2023
000000000-KWF5G_l01_n01_ELP4H3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-KWF5G_l01_n01_ELP4H3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3516
Sequences flagged as poor quality0
Sequence length301
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATGAGTCAGCGTCGAACATGCAACATTTATTGCATGATACGTGGCATTT325892.66211604095564No Hit
GATGAGTCAGCGTCNAACATGCAACATTTATTGCATGATACGTGGCATTT972.758816837315131No Hit
GATGAGTCAGCGTCGCACATGCAACATTTATTGCATGATACGTGGCATTT80.22753128555176336No Hit
ATGAGTCAGCGTCGAACATGCAACATTTATTGCATGATACGTGGCATTTC80.22753128555176336No Hit
GATGAGTCAGCGTCGAACATGCAACATTTCTTGCATGATACGTGGCATTT60.17064846416382254No Hit
GATGAGTCAGCGTCGAACATGCAACATTTATTGCATGATACGTGGCCTTT60.17064846416382254No Hit
GATGAGTCAGCGTCGAACATGCAACCTTTATTGCATGATACGTGGCATTT60.17064846416382254No Hit
GATGAGTCAGCGTCGACCATGCAACATTTATTGCATGATACGTGGCATTT50.1422070534698521No Hit
GATGAGTCAGCGTCGAAAATGCAACATTTATTGCATGATACGTGGCATTT40.11376564277588168No Hit
GATGAGTCAGCGTCGAACATGCAACATTTATTGCATGCTACGTGGCATTT40.11376564277588168No Hit
GATGAGTAAGCGTCGAACATGCAACATTTATTGCATGATACGTGGCATTT40.11376564277588168No Hit
GATGAGTCAGCGTCGAACATGCAACATTTATTGAATGATACGTGGCATTT40.11376564277588168No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)