FastQCFastQC Report
Mon 17 Apr 2023
000000000-KWF5G_l01_n01_ELP4H11.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-KWF5G_l01_n01_ELP4H11.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3555
Sequences flagged as poor quality0
Sequence length301
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATGAGTCAGCGTCGAACATGCAACATTTATTGCATGATACGTGGCATTT328692.43319268635724No Hit
GATGAGTCAGCGTCNAACATGCAACATTTATTGCATGATACGTGGCATTT932.6160337552742616No Hit
GATGAGTCAGCGTCGAACATGCAACATTTATTGCATGATACGTGGCCTTT90.25316455696202533No Hit
GATGAGTCAGCGTCGAACATGCACCATTTATTGCATGATACGTGGCATTT70.19690576652601968No Hit
ATGAGTCAGCGTCGAACATGCAACATTTATTGCATGATACGTGGCATTTC70.19690576652601968No Hit
GATGGTCAGCGTCGAACATGCAACATTTATTGCATGATACGTGGCATTTC50.14064697609001406No Hit
GTGAGTCAGCGTCGAACATGCAACATTTATTGCATGATACGTGGCATTTC50.14064697609001406No Hit
GATGAGTCAGCGTCGAACATGCAACATTTATTGCATGATAAGTGGCATTT50.14064697609001406No Hit
GATGAGTCAGCGTTGAACATGCAACATTTATTGCATGATACGTGGCATTT50.14064697609001406No Hit
GATGAGTCAGCGTCGCACATGCAACATTTATTGCATGATACGTGGCATTT40.11251758087201125No Hit
GATGAGTCAGCGTCGAACATGCAACCTTTATTGCATGATACGTGGCATTT40.11251758087201125No Hit
GATGAGTAAGCGTCGAACATGCAACATTTATTGCATGATACGTGGCATTT40.11251758087201125No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)