FastQCFastQC Report
Mon 17 Apr 2023
000000000-KWF5G_l01_n01_ELP4G5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-KWF5G_l01_n01_ELP4G5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6471
Sequences flagged as poor quality0
Sequence length301
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATGAGTCAGCGTCGAACATGCAACATTTATTGCATGATACGTGGCATTT575088.85798176479679No Hit
GATGAGTCAGCGTCNAACATGCAACATTTATTGCATGATACGTGGCATTT1702.6271055478287746No Hit
GATGAGTCAGCGTCGAACATGCAACATTTATTGCATGATACGTGGCATGT300.4636068613815485No Hit
GATGAGTCAGCGTCGAACATGCAACATTTATTGCATGATACGTGGCCTTT170.2627105547828774No Hit
GATGAGTCAGCGTCGAACATGCAACATTTATTGCATGATACGTGGCAGTT160.24725699273682586No Hit
GATGAGTCAGCGTCGAACATGCAACATTTATTGCATGATACGTGGCAATT160.24725699273682586No Hit
GATGAGTCAGCGTCGAACATGCAACCTTTATTGCATGATACGTGGCATTT150.23180343069077425No Hit
GTGAGTCAGCGTCGAACATGCAACATTTATTGCATGATACGTGGCATTTC140.2163498686447226No Hit
GATGAGTCAGCGTCGCACATGCAACATTTATTGCATGATACGTGGCATTT120.18544274455261936No Hit
GATGAGTCAGCGTCGAACATGCAACATTTATTGCATGATACGTTGCATTT90.13908205841446453No Hit
GATGAGTCAGCGTCGAACATGCAACATTTATTGCATGATAAGTGGCATTT90.13908205841446453No Hit
GATGGTCAGCGTCGAACATGCAACATTTATTGCATGATACGTGGCATTTC90.13908205841446453No Hit
GATGAGTAAGCGTCGAACATGCAACATTTATTGCATGATACGTGGCATTT80.12362849636841293No Hit
ATGAGTCAGCGTCGAACATGCAACATTTATTGCATGATACGTGGCATTTC80.12362849636841293No Hit
GATGATCAGCGTCGAACATGCAACATTTATTGCATGATACGTGGCATTTC70.1081749343223613No Hit
GATGAGTCAGCGTCGAACATGCATCATTTATTGCATGATACGTGGCATTT70.1081749343223613No Hit
GATGAGTCAGCGTCGAACATGCAACATTTCTTGCATGATACGTGGCATTT70.1081749343223613No Hit
GATGAGTCAGCGTCGAACATGCAACATATATTGCATGATACGTGGCATTT70.1081749343223613No Hit
GATGAGTCAGCGTCGAACATGCAACATTTATTGCATGATACGTGGCATTG70.1081749343223613No Hit
GATGAGTCAGCGTCGAACATGCCACATTTATTGCATGATACGTGGCATTT70.1081749343223613No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)