FastQCFastQC Report
Mon 17 Apr 2023
000000000-KWF5G_l01_n01_ELP4D4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-KWF5G_l01_n01_ELP4D4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5507
Sequences flagged as poor quality0
Sequence length301
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATGAGTCAGCGTCGAACATGCAACATTTATTGCATGATACGTGGCATTT496190.08534592337026No Hit
GATGAGTCAGCGTCNAACATGCAACATTTATTGCATGATACGTGGCATTT1813.2867259851098605No Hit
ATGAGTCAGCGTCGAACATGCAACATTTATTGCATGATACGTGGCATTTC150.27238060650081714No Hit
GATGAGTCAGCGTCGAACATGCAACATTTATTGCATGATAAGTGGCATTT90.1634283639004903No Hit
GATGAGTCAGGTCGAACATGCAACATTTATTGCATGATACGTGGCATTTC90.1634283639004903No Hit
GATGAGTCAGCGTCGAACATGCAACATTTATTGCATGATACGTGGCATGT90.1634283639004903No Hit
GATGAGTCAGCGTCGAACATGCAACATTTATTGCATGATACGTGGCATCT80.1452696568004358No Hit
GATGAGTCAGCGTCGAACATGCAACCTTTATTGCATGATACGTGGCATTT80.1452696568004358No Hit
GATGAGTCAGCGTCGAACATGCAACATTGATTGCATGATACGTGGCATTT70.12711094970038134No Hit
GATGAGTCAGCGTCGAACATGCAACATGTATTGCATGATACGTGGCATTT70.12711094970038134No Hit
GATGAGTCAGCGTCGAACATGCAACATTTATTGCATGATACGTGGCAATT70.12711094970038134No Hit
GATGAGTCAGCGTCGAACATGCAACATTTATTACATGATACGTGGCATTT60.10895224260032686No Hit
GATGAGTCAGCGTCGAACATGCAACATTTATTGCATGATACGTGGCAGTT60.10895224260032686No Hit
GATGAGTCAGCGTCGAACATGCAACATTTATTGCATGATACGTGGAATTT60.10895224260032686No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)