FastQCFastQC Report
Mon 17 Apr 2023
000000000-KWF5G_l01_n01_ELP4C10.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-KWF5G_l01_n01_ELP4C10.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4656
Sequences flagged as poor quality0
Sequence length301
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATGAGTCAGCGTCGAACATGCAACATTTATTGCATGATACGTGGCATTT433393.0627147766323No Hit
GATGAGTCAGCGTCNAACATGCAACATTTATTGCATGATACGTGGCATTT1152.4699312714776633No Hit
GATGAGTCAGCGTCGAACATGCCACATTTATTGCATGATACGTGGCATTT100.2147766323024055No Hit
GTGAGTCAGCGTCGAACATGCAACATTTATTGCATGATACGTGGCATTTC70.15034364261168387No Hit
GATGAGTCAGCGTCGAACATGCAACATTTATTGCATGATACGTGGCCTTT60.12886597938144329No Hit
GATGGTCAGCGTCGAACATGCAACATTTATTGCATGATACGTGGCATTTC60.12886597938144329No Hit
GATGAGTCAGCGTCTAACATGCAACATTTATTGCATGATACGTGGCATTT60.12886597938144329No Hit
GATGAGTCAGCGTCGAACATGCAACCTTTATTGCATGATACGTGGCATTT50.10738831615120276No Hit
GATGAGTCAGCGTCGAACCTGCAACATTTATTGCATGATACGTGGCATTT50.10738831615120276No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)