FastQCFastQC Report
Mon 17 Apr 2023
000000000-KWF5G_l01_n01_ELP4B1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-KWF5G_l01_n01_ELP4B1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3885
Sequences flagged as poor quality0
Sequence length301
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATGAGTCAGCGTCGAACATGCAACATTTATTGCATGATACGTGGCATTT360592.7927927927928No Hit
GATGAGTCAGCGTCNAACATGCAACATTTATTGCATGATACGTGGCATTT962.471042471042471No Hit
GTGAGTCAGCGTCGAACATGCAACATTTATTGCATGATACGTGGCATTTC70.18018018018018017No Hit
ATGAGTCAGCGTCGAACATGCAACATTTATTGCATGATACGTGGCATTTC70.18018018018018017No Hit
GATGAGTCAGCGTCGAACATGCAACATTTCTTGCATGATACGTGGCATTT60.15444015444015444No Hit
GATGAGTCAGCTCGAACATGCAACATTTATTGCATGATACGTGGCATTTC60.15444015444015444No Hit
GATGAGTCAGCGTCGAACATGCAACATTTATTGCATGATAAGTGGCATTT60.15444015444015444No Hit
GATGAGTCAGCGTCGCACATGCAACATTTATTGCATGATACGTGGCATTT50.1287001287001287No Hit
GATGAGTCAGCGTCGAACATGCAACATTTATTGCCTGATACGTGGCATTT50.1287001287001287No Hit
TATGAGTCAGCGTCGAACATGCAACATTTATTGCATGATACGTGGCATTT50.1287001287001287No Hit
GATGAGTCAGCGTCGAACATGCAACATTTATTGCATGCTACGTGGCATTT50.1287001287001287No Hit
GATGAGTCAGCGTCGAACATGCAACCTTTATTGCATGATACGTGGCATTT40.10296010296010295No Hit
GCTGAGTCAGCGTCGAACATGCAACATTTATTGCATGATACGTGGCATTT40.10296010296010295No Hit
GATGAGTCAGCGTCGAACATGCACCATTTATTGCATGATACGTGGCATTT40.10296010296010295No Hit
GATGAGTCAGCGTCAAACATGCAACATTTATTGCATGATACGTGGCATTT40.10296010296010295No Hit
GATGAGTCACGTCGAACATGCAACATTTATTGCATGATACGTGGCATTTC40.10296010296010295No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)