FastQCFastQC Report
Mon 17 Apr 2023
000000000-KWF5G_l01_n01_ELP4B11.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-KWF5G_l01_n01_ELP4B11.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4704
Sequences flagged as poor quality0
Sequence length301
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATGAGTCAGCGTCGAACATGCAACATTTATTGCATGATACGTGGCATTT437593.00595238095238No Hit
GATGAGTCAGCGTCNAACATGCAACATTTATTGCATGATACGTGGCATTT1052.232142857142857No Hit
GATGAGTCAGCGTCGCACATGCAACATTTATTGCATGATACGTGGCATTT140.2976190476190476No Hit
ATGAGTCAGCGTCGAACATGCAACATTTATTGCATGATACGTGGCATTTC90.1913265306122449No Hit
GATGAGTCAGCGTCGAACATGCAACCTTTATTGCATGATACGTGGCATTT80.17006802721088435No Hit
GATGAGTCAGCGTCGAACATGCAACATTTATTGCATGATACGTGGCCTTT70.1488095238095238No Hit
GTGAGTCAGCGTCGAACATGCAACATTTATTGCATGATACGTGGCATTTC70.1488095238095238No Hit
GATGAGTCAGCGTCGAACATGCACCATTTATTGCATGATACGTGGCATTT60.12755102040816327No Hit
GATGAGTCAGCGTCGACCATGCAACATTTATTGCATGATACGTGGCATTT60.12755102040816327No Hit
GATGAGTCAGCGTCTAACATGCAACATTTATTGCATGATACGTGGCATTT50.10629251700680273No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)