FastQCFastQC Report
Mon 17 Apr 2023
000000000-KWF5G_l01_n01_ELP3G4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-KWF5G_l01_n01_ELP3G4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4042
Sequences flagged as poor quality0
Sequence length301
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATGAGTCAGCGTCGAACATGCAACATTTATTGCATGATACGTGGCATTT364990.27709054923305No Hit
GATGAGTCAGCGTCNAACATGCAACATTTATTGCATGATACGTGGCATTT932.300841167738743No Hit
GATGAGTCAGCGTCGAACATGCAACATTTATTGCATGATACGTGGCATGT160.3958436417615042No Hit
ATGAGTCAGCGTCGAACATGCAACATTTATTGCATGATACGTGGCATTTC110.2721425037110341No Hit
GATGAGTCAGCGTCGCACATGCAACATTTATTGCATGATACGTGGCATTT70.17318159327065807No Hit
GTGAGTCAGCGTCGAACATGCAACATTTATTGCATGATACGTGGCATTTC70.17318159327065807No Hit
GATGAGTCAGCGTCGAACATGCAACCTTTATTGCATGATACGTGGCATTT60.14844136566056407No Hit
GATGAGTCAGCGTCGAACATGCAACATTTATTGCATGATAAGTGGCATTT60.14844136566056407No Hit
GATGAGTCAGCGTCGAACATGCAACATTTATTGCATGATACGTGGCCTTT50.12370113805047006No Hit
GATGAGTCAGGTCGAACATGCAACATTTATTGCATGATACGTGGCATTTC50.12370113805047006No Hit
GATGAGTCAGCGTCGAACATGCAACATGTATTGCATGATACGTGGCATTT50.12370113805047006No Hit
GATGAGTCAGCGTCGAACATGCAACATTTATTGCATGATACGTGGCAATT50.12370113805047006No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)