FastQCFastQC Report
Mon 17 Apr 2023
000000000-KWF5G_l01_n01_ELP3G10.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-KWF5G_l01_n01_ELP3G10.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5634
Sequences flagged as poor quality0
Sequence length301
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATGAGTCAGCGTCGAACATGCAACATTTATTGCATGATACGTGGCATTT520992.45651402200923No Hit
GATGAGTCAGCGTCNAACATGCAACATTTATTGCATGATACGTGGCATTT1532.7156549520766773No Hit
ATGAGTCAGCGTCGAACATGCAACATTTATTGCATGATACGTGGCATTTC120.21299254526091588No Hit
GATGATCAGCGTCGAACATGCAACATTTATTGCATGATACGTGGCATTTC110.1952431664891729No Hit
GTGAGTCAGCGTCGAACATGCAACATTTATTGCATGATACGTGGCATTTC110.1952431664891729No Hit
GATGAGTCAGCGTCGAACATGCAACATTTATTGCATGATACGTGGCCTTT100.1774937877174299No Hit
GATGAGTCAGCGTCGAACATGCAACATTTATTGCATGCTACGTGGCATTT100.1774937877174299No Hit
GATGAGTCAGCGTCGAACATGCAACCTTTATTGCATGATACGTGGCATTT80.1419950301739439No Hit
GATGAGTCAGCGTCGAACATGCAACATTTATTGCATGATAAGTGGCATTT80.1419950301739439No Hit
GATGAGTCAGCGTCGAACATGCAACATTTATTGCATGATACGTTGCATTT70.12424565140220092No Hit
GATGAGTCAGCGTCGCACATGCAACATTTATTGCATGATACGTGGCATTT60.10649627263045794No Hit
GATGAGTCAGCTCGAACATGCAACATTTATTGCATGATACGTGGCATTTC60.10649627263045794No Hit
GATGAGTCAGCGTCGAACCTGCAACATTTATTGCATGATACGTGGCATTT60.10649627263045794No Hit
GATGAGTCAGCGTCGAACATGCACCATTTATTGCATGATACGTGGCATTT60.10649627263045794No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)