FastQCFastQC Report
Mon 17 Apr 2023
000000000-KWF5G_l01_n01_ELP3F8.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-KWF5G_l01_n01_ELP3F8.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5151
Sequences flagged as poor quality0
Sequence length301
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATGAGTCAGCGTCGAACATGCAACATTTATTGCATGATACGTGGCATTT452187.7693651718113No Hit
GATGAGTCAGCGTCNAACATGCAACATTTATTGCATGATACGTGGCATTT1122.17433508056688No Hit
GACTACAACCGGATTAGATACCCTGGTAGTCCACGCCGTAAACGATGAGT190.36886041545331005No Hit
GATGAGTCAGCGTCGAACATGCAACATTTATTGCATGATACGTGGCATGT180.34944670937682004No Hit
GATGAGTCAGCGTCGAACATGCAACATTTATTGCATGATAAGTGGCATTT150.29120559114735No Hit
GATGAGTCAGCGTCGAACATGCAACATTTATTGCATGATACGTGGCAGTT130.25237817899437004No Hit
ATGAGTCAGCGTCGAACATGCAACATTTATTGCATGATACGTGGCATTTC130.25237817899437004No Hit
GATGAGTCAGCGTCGCACATGCAACATTTATTGCATGATACGTGGCATTT120.23296447291788003No Hit
GATGAGTCAGCGTCGAACATGCAACATTTATTGCATGATACGTGGCAATT110.21355076684139002No Hit
GACTACAACAGGATTAGATACCCTGGTAGTCCACGCCGTAAACGATGAGT90.17472335468841002No Hit
GTGAGTCAGCGTCGAACATGCAACATTTATTGCATGATACGTGGCATTTC90.17472335468841002No Hit
GATGAGTCAGCGTCGAACATGCAACATTTATTGCATGATACGTGGCCTTT70.13589594253543No Hit
GATGAGTAAGCGTCGAACATGCAACATTTATTGCATGATACGTGGCATTT70.13589594253543No Hit
GATGAGTCAGCGTCGAACATGCACCATTTATTGCATGATACGTGGCATTT70.13589594253543No Hit
GATGCGTCAGCGTCGAACATGCAACATTTATTGCATGATACGTGGCATTT70.13589594253543No Hit
GATGAGTCAGCGTCGAACATGCAACATTTATTGCATGATACGAGGCATTT60.11648223645894001No Hit
GATGAGTCAGCGTCGAACATGCAACATTTATTGCATGATACGTGGCATAT60.11648223645894001No Hit
GATGAGTCAGCGTCGAACATGCAACATATATTGCATGATACGTGGCATTT60.11648223645894001No Hit
GATGAGTCAGCGTCGAACATGCAACCTTTATTGCATGATACGTGGCATTT60.11648223645894001No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)