FastQCFastQC Report
Mon 17 Apr 2023
000000000-KWF5G_l01_n01_ELP3F4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-KWF5G_l01_n01_ELP3F4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5120
Sequences flagged as poor quality0
Sequence length301
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATGAGTCAGCGTCGAACATGCAACATTTATTGCATGATACGTGGCATTT450688.0078125No Hit
GATGAGTCAGCGTCNAACATGCAACATTTATTGCATGATACGTGGCATTT1332.59765625No Hit
GATGAGTCAGCGTCGAACATGCAACATTTATTGCATGATACGTGGCATGT160.3125No Hit
GACTACAACAGGATTAGATACCCTGGTAGTCCACGCCGTAAACGATGAGT140.2734375No Hit
GATGAGTCAGCGTCGCACATGCAACATTTATTGCATGATACGTGGCATTT130.25390625No Hit
GACTACAACCGGATTAGATACCCTGGTAGTCCACGCCGTAAACGATGAGT130.25390625No Hit
GATGAGTCAGCGTCGAACATGCAACATTTATTGCATGATAAGTGGCATTT120.234375No Hit
GATGAGTCAGCGTCGAACATGCAACATTTATTGCATGATACGTGGCAGTT110.21484375000000003No Hit
GATGAGTCAGCGTCGAACATGCAACATTTATTGCATGATACGTGGCCTTT100.1953125No Hit
GATGAGTCAGCGTCGAACATGCAACATTTATTGCATGATACGGGGCATTT100.1953125No Hit
GTGAGTCAGCGTCGAACATGCAACATTTATTGCATGATACGTGGCATTTC80.15625No Hit
ATGAGTCAGCGTCGAACATGCAACATTTATTGCATGATACGTGGCATTTC80.15625No Hit
GATGAGTCAGCGTCGAACATGCAACATTTATTGCATGCTACGTGGCATTT80.15625No Hit
GATGCGTCAGCGTCGAACATGCAACATTTATTGCATGATACGTGGCATTT80.15625No Hit
GATGAGTCAGCGTCGAACATGCAACCTTTATTGCATGATACGTGGCATTT70.13671875No Hit
GATGAGTCCGCGTCGAACATGCAACATTTATTGCATGATACGTGGCATTT70.13671875No Hit
GATGAGTCAGCGTCGAACATGCAACATTTATTGCATGATACGTGGCAATT70.13671875No Hit
GATGAGTCAGCGTCGAACATGCAACATTTATTGCATGATACGTAGCATTT60.1171875No Hit
GATGAGTCAGCGTCGAACCTGCAACATTTATTGCATGATACGTGGCATTT60.1171875No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)