FastQCFastQC Report
Mon 17 Apr 2023
000000000-KWF5G_l01_n01_ELP3E8.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-KWF5G_l01_n01_ELP3E8.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4787
Sequences flagged as poor quality0
Sequence length301
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATGAGTCAGCGTCGAACATGCAACATTTATTGCATGATACGTGGCATTT384780.36348443701692No Hit
GACTACAACCGGATTAGATACCCTGGTAGTCCACGCCGTAAACGATGAGT1222.548569041153123No Hit
GACTACAACAGGATTAGATACCCTGGTAGTCCACGCCGTAAACGATGAGT1172.444119490286192No Hit
GATGAGTCAGCGTCNAACATGCAACATTTATTGCATGATACGTGGCATTT1012.1098809275120116No Hit
GATGAGTCAGCGTCGAACATGCAACATTTATTGCATGATAAGTGGCATTT290.6058073950282014No Hit
ATGAGTCAGCGTCGAACATGCAACATTTATTGCATGATACGTGGCATTTC180.3760183831209526No Hit
GACTACAACCGGATTAGATACCCTGGTAGTCCACGCCGTAAACGATGTCA180.3760183831209526No Hit
GATGAGTCAGCGTCGCACATGCAACATTTATTGCATGATACGTGGCATTT160.33423856277418007No Hit
GACTACAACAGGATTAGATACCCTGGTAGTCCACGCCGTAAACGATGTCA110.2297890119072488No Hit
GATGAGTCAGCGTCGAACATGCAACATTTATTGCATGCTACGTGGCATTT90.1880091915604763No Hit
GATGAGTCAGCGTCGAACATGCAACATTTATTGCATGATACGTGGCCTTT80.16711928138709004No Hit
GATGAGTCAGCGTCGAACATGCAACATTTATTGCCTGATACGTGGCATTT80.16711928138709004No Hit
GATGAGTCAGCGTCGAACATGCAACATTTATTGAATGATACGTGGCATTT80.16711928138709004No Hit
GATGAGTCAGCGTCGAACATGCAACATTGATTGCATGATACGTGGCATTT70.14622937121370377No Hit
GATGAGTCAGCGTCGAACATGCAACCTTTATTGCATGATACGTGGCATTT70.14622937121370377No Hit
GACTACAACCGGATTAGCTACCCTGGTAGTCCACGCCGTAAACGATGAGT70.14622937121370377No Hit
GATGAGTCAGCGTCGAACATGCAACATTTATTGCATGATACGGGGCATTT60.1253394610403175No Hit
GATGAGTCAGCGTCGAACATGCAACATTTCTTGCATGATACGTGGCATTT60.1253394610403175No Hit
GATGAGTCAGCGTCGAACATGCAACATTTATTGCATGATACGTGGCATTA60.1253394610403175No Hit
GATGAGTCAGCGACGAACATGCAACATTTATTGCATGATACGTGGCATTT60.1253394610403175No Hit
GACTACAACCGGATTAGATACCCTGGTATTCCACGCCGTAAACGATGAGT50.10444955086693128No Hit
GACTACAACAGGATTAGATACCCTGGTAGTCCACGCCGTACACGATGAGT50.10444955086693128No Hit
GATGAGTCAGCGTCGAACATGCAACATTTATTGCATGATACGTGGCAGTT50.10444955086693128No Hit
GACTACAACCGGATTAGATACCCTGGTAGTCCACGCCGTACACGATGAGT50.10444955086693128No Hit
GATGAGTCAGCGTCGAACATGCAACATTTATTGCATGATACGTGGCATGT50.10444955086693128No Hit
GATGAGTCAGCGTCGAACATGCAACATTTATTGCATGATACGTGGAATTT50.10444955086693128No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)