FastQCFastQC Report
Mon 17 Apr 2023
000000000-KWF5G_l01_n01_ELP3D12.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-KWF5G_l01_n01_ELP3D12.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5418
Sequences flagged as poor quality0
Sequence length301
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATGAGTCAGCGTCGAACATGCAACATTTATTGCATGATACGTGGCATTT484589.42414174972313No Hit
GATGAGTCAGCGTCNAACATGCAACATTTATTGCATGATACGTGGCATTT1262.3255813953488373No Hit
GATGAGTCAGCGTCGAACATGCAACATTTATTGCATGATACGTGGCATGT270.4983388704318937No Hit
GATGAGTCAGCGTCGAACATGCAACATTTATTGCATGATACGTGGCAGTT160.29531192321889993No Hit
ATGAGTCAGCGTCGAACATGCAACATTTATTGCATGATACGTGGCATTTC140.2583979328165375No Hit
GATGAGTCAGCGTCGAACATGCAACATTTATTGCATGATAAGTGGCATTT120.22148394241417496No Hit
GATGAGTCAGCGTCGAACATGCAACCTTTATTGCATGATACGTGGCATTT120.22148394241417496No Hit
GATGAGTCAGCGTCGAACATGCAACATTTATTGCATGATACGTGGCCTTT110.2030269472129937No Hit
GATGAGTCAGCGTCGAACATGCAACATTGATTGCATGATACGTGGCATTT90.16611295681063123No Hit
GATGAGTCAGCGTCGAACATGCAACATTTATTGCATGATACGTGGCATTG90.16611295681063123No Hit
GATGAGTCAGCGTCGAACATGCAACATTTCTTGCATGATACGTGGCATTT80.14765596160944996No Hit
GATGAGTCAGCGTCGAACATGCAACATTTATTGCATGATACGTGGCAATT80.14765596160944996No Hit
GATGAGTCAGCGTCGAACATGCAACATTTATTGCATGCTACGTGGCATTT70.12919896640826875No Hit
GATGATCAGCGTCGAACATGCAACATTTATTGCATGATACGTGGCATTTC60.11074197120708748No Hit
GATGAGTCAGCGTCGAACATGCAACATTTATTACATGATACGTGGCATTT60.11074197120708748No Hit
GATGAGTCAGCGTCGAACCTGCAACATTTATTGCATGATACGTGGCATTT60.11074197120708748No Hit
GATGCGTCAGCGTCGAACATGCAACATTTATTGCATGATACGTGGCATTT60.11074197120708748No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)