FastQCFastQC Report
Mon 17 Apr 2023
000000000-KWF5G_l01_n01_ELP3C5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-KWF5G_l01_n01_ELP3C5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5971
Sequences flagged as poor quality0
Sequence length301
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATGAGTCAGCGTCGAACATGCAACATTTATTGCATGATACGTGGCATTT532889.23128454195277No Hit
GATGAGTCAGCGTCNAACATGCAACATTTATTGCATGATACGTGGCATTT1722.8805895159939707No Hit
GATGAGTCAGCGTCGAACATGCAACATTTATTGCATGATACGTGGCATGT230.3851951096968682No Hit
GATGAGTCAGCGTCGAACATGCAACATTTATTGCATGATAAGTGGCATTT160.2679618154412996No Hit
GATGAGTCAGCGTCGAACATGCAACATTTATTGCATGATACGGGGCATTT110.18422374811589348No Hit
GATGAGTCAGCGTCGAACATGCAACATTTATTGCATGATACGTGGCAGTT110.18422374811589348No Hit
GATGAGTCAGCGTCGCACATGCAACATTTATTGCATGATACGTGGCATTT110.18422374811589348No Hit
GATGAGTCAGCGTCGAACATGCAACATTGATTGCATGATACGTGGCATGT100.16747613465081226No Hit
GATGAGTCAGCGTCGAACATGCAACATTTATTGCATGATACGTGGCATTG90.15072852118573102No Hit
GATGAGTCAGCGTCGAACATGCAACATTTATTGCATGCTACGTGGCATTT90.15072852118573102No Hit
GATGAGTCAGCGTCGAACATGCAACATTTATTGCATGATACGTGGCCTTT80.1339809077206498No Hit
GATGAGTAAGCGTCGAACATGCAACATTTATTGCATGATACGTGGCATTT80.1339809077206498No Hit
GATGAGTCAGCGTCGAACATGCAACATTGATTGCATGATACGTGGCATTT80.1339809077206498No Hit
GATGAGTCAGCGTCGAACATGCACCATTTATTGCATGATACGTGGCATTT70.11723329425556857No Hit
ATGAGTCAGCGTCGAACATGCAACATTTATTGCATGATACGTGGCATTTC70.11723329425556857No Hit
GATGAGTCAGCGTCGAACATGCAACATATATTGCATGATACGTGGCATTT70.11723329425556857No Hit
GATGAGTCAGCGTCGAACATGCAACATTTATTGCCTGATACGTGGCATTT60.10048568079048736No Hit
GATGAGTCAGCGTCGAACATGCAACATGTATTGCATGATACGTGGCATGT60.10048568079048736No Hit
GATGAGTCAGCGTCGAACATGCAACCTTTATTGCATGATACGTGGCATTT60.10048568079048736No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)