FastQCFastQC Report
Mon 17 Apr 2023
000000000-KWF5G_l01_n01_ELP3B8.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-KWF5G_l01_n01_ELP3B8.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5573
Sequences flagged as poor quality0
Sequence length301
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATGAGTCAGCGTCGAACATGCAACATTTATTGCATGATACGTGGCATTT499089.53884801722592No Hit
GATGAGTCAGCGTCNAACATGCAACATTTATTGCATGATACGTGGCATTT1482.6556612237574018No Hit
GATGAGTCAGCGTCGAACATGCAACATTTATTGCATGATACGTGGCATGT150.2691548537591961No Hit
ATGAGTCAGCGTCGAACATGCAACATTTATTGCATGATACGTGGCATTTC120.2153238830073569No Hit
GATGAGTCAGCGTCGCACATGCAACATTTATTGCATGATACGTGGCATTT120.2153238830073569No Hit
GTGAGTCAGCGTCGAACATGCAACATTTATTGCATGATACGTGGCATTTC110.19738022609007716No Hit
GATGAGTCAGCGTCGAACATGCAACATTTATTGCATGATACGTGGCATTG110.19738022609007716No Hit
GATGAGTCAGCGTCGAACATGCAACATTTATTGCATGATAAGTGGCATTT90.16149291225551768No Hit
GATGAGTCAGCGTCGAACATGCAACATTTATTGCATGATACGTGGCAATT90.16149291225551768No Hit
GATGAGTCAGCGTCGAACATGCAACATTTATTGCATGATACGGGGCATTT80.14354925533823795No Hit
GATGAGTCAGCGTCGAACATGCAACATTTATTGCATGATACGTGGCCTTT70.1256055984209582No Hit
GATGAGTCAGCGTCGAACATGCAACATTTATTGCATGATACGTGGCAGTT70.1256055984209582No Hit
GATGAGTCAGCGTCGAACATGCAACATTTCTTGCATGATACGTGGCATTT70.1256055984209582No Hit
GATGAGTCAGCGTCGAACATGCAACATTGATTGCATGATACGTGGCATTT60.10766194150367844No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)