FastQCFastQC Report
Mon 17 Apr 2023
000000000-KWF5G_l01_n01_ELP3B4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-KWF5G_l01_n01_ELP3B4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5724
Sequences flagged as poor quality0
Sequence length301
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATGAGTCAGCGTCGAACATGCAACATTTATTGCATGATACGTGGCATTT518990.6533892382949No Hit
GATGAGTCAGCGTCNAACATGCAACATTTATTGCATGATACGTGGCATTT1402.4458420684835778No Hit
GATGAGTCAGCGTCGAACATGCAACATTGATTGCATGATACGTGGCATTT120.20964360587002098No Hit
ATGAGTCAGCGTCGAACATGCAACATTTATTGCATGATACGTGGCATTTC110.19217330538085253No Hit
GTGAGTCAGCGTCGAACATGCAACATTTATTGCATGATACGTGGCATTTC100.17470300489168414No Hit
GATGAGTCAGCGTCGAACATGCAACATTTATTGCATGATAAGTGGCATTT100.17470300489168414No Hit
GATGAGTCAGCGTCGAACATGCAACATTTATTGCATGATACGTGGCATGT90.15723270440251574No Hit
GATGAGTCAGCGTCGAACATGCAACATTTATTGCATGATACGTGGCAATT90.15723270440251574No Hit
GATGAGTCAGCGTCGAACATGCAACATTTATTGCATGATACGGGGCATTT70.1222921034241789No Hit
GATGAGTCAGCGTCGAACATGCAACATTTATTGCATGATCCGTGGCATTT70.1222921034241789No Hit
GATGAGTCAGCGTCGACCATGCAACATTTATTGCATGATACGTGGCATTT70.1222921034241789No Hit
GATGAGTCAGCGTCGAACATGCAACATTTATTGCATGATACGTGGCAGTT60.10482180293501049No Hit
GATGAGTCAGCGTCGAACATGCAACATATATTGCATGATACGTGGCATTT60.10482180293501049No Hit
GATGAGTCAGCGTCGAACATGCCACATTTATTGCATGATACGTGGCATTT60.10482180293501049No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)