FastQCFastQC Report
Mon 17 Apr 2023
000000000-KWF5G_l01_n01_ELP3A6.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-KWF5G_l01_n01_ELP3A6.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5326
Sequences flagged as poor quality0
Sequence length301
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATGAGTCAGCGTCGAACATGCAACATTTATTGCATGATACGTGGCATTT493992.73375891851295No Hit
GATGAGTCAGCGTCNAACATGCAACATTTATTGCATGATACGTGGCATTT1522.853924145700338No Hit
ATGAGTCAGCGTCGAACATGCAACATTTATTGCATGATACGTGGCATTTC150.2816372512204281No Hit
GACTACAACAGGATTAGATACCCTGGTAGTCCACGCCCTAAACGATGTCA80.1502065339842283No Hit
GATGAGTCAGCGTCGAACATGCAACCTTTATTGCATGATACGTGGCATTT70.13143071723619978No Hit
GATGAGTCAGCGTCGAACATGCAACATTTATTGCATGCTACGTGGCATTT70.13143071723619978No Hit
GATGAGTCAGCGTCGCACATGCAACATTTATTGCATGATACGTGGCATTT60.11265490048817123No Hit
GATGAGTCAGCGTCGAACATGCAACATTTATTGCATGATACGTGGCCTTT60.11265490048817123No Hit
GATGAGTCAGCGTCGAATATGCAACATTTATTGCATGATACGTGGCATTT60.11265490048817123No Hit
GTGAGTCAGCGTCGAACATGCAACATTTATTGCATGATACGTGGCATTTC60.11265490048817123No Hit
GATGAGTCAGCGTCGAACATGCAACATTTATTGCATGATAAGTGGCATTT60.11265490048817123No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)