FastQCFastQC Report
Mon 17 Apr 2023
000000000-KWF5G_l01_n01_ELP3A3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-KWF5G_l01_n01_ELP3A3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4986
Sequences flagged as poor quality0
Sequence length301
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATGAGTCAGCGTCGAACATGCAACATTTATTGCATGATACGTGGCATTT440788.38748495788207No Hit
GATGAGTCAGCGTCNAACATGCAACATTTATTGCATGATACGTGGCATTT1062.125952667468913No Hit
GACTACAACCGGATTAGATACCCTGGTAGTCCACGCCGTAAACGATGAGT851.704773365423185No Hit
GACTACAACAGGATTAGATACCCTGGTAGTCCACGCCGTAAACGATGAGT741.4841556357801846No Hit
GACTACAACCGGATTAGATACCCTGGTAGTCCACGCCGTAAACGATGTCA280.5615724027276374No Hit
GACTACAACAGGATTAGATACCCTGGTAGTCCACGCCGTAAACGATGTCA260.5214600882470918No Hit
GATGAGTCAGCGTCGAACATGCAACATTTATTGCATGATACGTGGCCTTT110.2206177296430004No Hit
GATGAGTCAGCGTCGCACATGCAACATTTATTGCATGATACGTGGCATTT100.20056157240272765No Hit
ATGAGTCAGCGTCGAACATGCAACATTTATTGCATGATACGTGGCATTTC90.18050541516245489No Hit
GATGAGTCAGCGTCTAACATGCAACATTTATTGCATGATACGTGGCATTT80.1604492579221821No Hit
GACTACAACCGGATNAGATACCCTGGTAGTCCACGCCGTAAACGATGAGT60.12033694344163659No Hit
GTGAGTCAGCGTCGAACATGCAACATTTATTGCATGATACGTGGCATTTC60.12033694344163659No Hit
GATGAGTCAGCGTCGAACATGCAACCTTTATTGCATGATACGTGGCATTT50.10028078620136383No Hit
GATGAGTCAGCGTCGAACATGCAACATTTATTGCATGCTACGTGGCATTT50.10028078620136383No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)