FastQCFastQC Report
Mon 17 Apr 2023
000000000-KWF5G_l01_n01_ELP3A1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-KWF5G_l01_n01_ELP3A1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2618
Sequences flagged as poor quality0
Sequence length301
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATGAGTCAGCGTCGAACATGCAACATTTATTGCATGATACGTGGCATTT242392.55156608097785No Hit
GATGAGTCAGCGTCNAACATGCAACATTTATTGCATGATACGTGGCATTT542.0626432391138274No Hit
GATGAGTCAGCGTCGCACATGCAACATTTATTGCATGATACGTGGCATTT60.2291825821237586No Hit
GATGGTCAGCGTCGAACATGCAACATTTATTGCATGATACGTGGCATTTC50.19098548510313218No Hit
GAGAGTCAGCGTCGAACATGCAACATTTATTGCATGATACGTGGCATTTC40.15278838808250572No Hit
GATGAGTCAGCGTCGAACATGCACCATTTATTGCATGATACGTGGCATTT40.15278838808250572No Hit
GATGCGTCAGCGTCGAACATGCAACATTTATTGCATGATACGTGGCATTT30.1145912910618793No Hit
GATGATCAGCGTCGAACATGCAACATTTATTGCATGATACGTGGCATTTC30.1145912910618793No Hit
GATGAGTCAGCGTCGAACATGCAACATTTATTGCATGATAAGTGGCATTT30.1145912910618793No Hit
GATGAGTCAGCGTCGAACATGCAACATTTATTGCATGATACGTGGCGTTT30.1145912910618793No Hit
GATGAGTCAGCGTCGAAAATGCAACATTTATTGCATGATACGTGGCATTT30.1145912910618793No Hit
GACTACAACAGGATTAGATACCCTGGTAGTCCACGCCGTAAACGATGAGT30.1145912910618793No Hit
GATGAGTCAGCGTCGAACATGCAACATTTATTGCATGATACGTGGCATCT30.1145912910618793No Hit
ATGAGTCAGCGTCGAACATGCAACATTTATTGCATGATACGTGGCATTTC30.1145912910618793No Hit
GACTACAACCGGATTAGATACCCTGGTAGTCCACGCCGTAAACGATGTCA30.1145912910618793No Hit
GATGAGTCAGGTCGAACATGCAACATTTATTGCATGATACGTGGCATTTC30.1145912910618793No Hit
GATGAGTCAGCGTCGAACCTGCAACATTTATTGCATGATACGTGGCATTT30.1145912910618793No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)