FastQCFastQC Report
Mon 17 Apr 2023
000000000-KWF5G_l01_n01_ELP3A10.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-KWF5G_l01_n01_ELP3A10.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5984
Sequences flagged as poor quality0
Sequence length301
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATGAGTCAGCGTCGAACATGCAACATTTATTGCATGATACGTGGCATTT559293.44919786096256No Hit
GATGAGTCAGCGTCNAACATGCAACATTTATTGCATGATACGTGGCATTT1522.5401069518716577No Hit
ATGAGTCAGCGTCGAACATGCAACATTTATTGCATGATACGTGGCATTTC170.2840909090909091No Hit
GATGAGTCAGCGTCGCACATGCAACATTTATTGCATGATACGTGGCATTT140.2339572192513369No Hit
GTGAGTCAGCGTCGAACATGCAACATTTATTGCATGATACGTGGCATTTC90.15040106951871657No Hit
GATGAGTCAGCGTCGACCATGCAACATTTATTGCATGATACGTGGCATTT80.1336898395721925No Hit
GATGAGTCAGCGTCGAACATGCAACATTTATTGCATGATACGTGGCCTTT70.11697860962566844No Hit
GATGAGTCAGCGTCGAACATGCCACATTTATTGCATGATACGTGGCATTT70.11697860962566844No Hit
GATGAGTCAGCGTCGAACATGCAACCTTTATTGCATGATACGTGGCATTT60.1002673796791444No Hit
GATGAGTCAGCGTCGAACATGCAACATTTATTGCATGCTACGTGGCATTT60.1002673796791444No Hit
GATGAGTCAGCGTCGAACATGCACCATTTATTGCATGATACGTGGCATTT60.1002673796791444No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)