FastQCFastQC Report
Mon 17 Apr 2023
000000000-KWF5G_l01_n01_ELP2H4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-KWF5G_l01_n01_ELP2H4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5171
Sequences flagged as poor quality0
Sequence length301
%GC53

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GACTACAACCGGATTAGATACCCTGGTAGTCCACGCCGTAAACGATGAGT150329.065944691549024No Hit
GACTACAACAGGATTAGATACCCTGGTAGTCCACGCCGTAAACGATGAGT119223.051634113324308No Hit
GACTACAACCGGATTAGATACCCTGGTAGTCCACGCCGTAAACGATGTCA78715.219493328176368No Hit
GACTACAACAGGATTAGATACCCTGGTAGTCCACGCCGTAAACGATGTCA75614.619996132276155No Hit
GACTACAACAGGATTAGATACCCTGGTAGTCCACGCCCTAAACGATGTCA881.7017984915877007No Hit
GACTACAACCGGATTAGATACCCTGGTAGTCCACGCCCTAAACGATGTCA801.5470895378070006No Hit
GACTACAACAGGATNAGATACCCTGGTAGTCCACGCCGTAAACGATGAGT360.6961902920131503No Hit
GACTACAACCGGATNAGATACCCTGGTAGTCCACGCCGTAAACGATGAGT320.6188358151228002No Hit
GACTACAACAGGATTAGATACCCTGGTAGTCCACGCCGTAAACGATGAAT220.4254496228969252No Hit
GACTACAACCGGATTAGATACCCTGGTAGTCCACGCCGTAAACGATGATA190.3674337652291626No Hit
GACTACAACCGGATNAGATACCCTGGTAGTCCACGCCGTAAACGATGTCA190.3674337652291626No Hit
GACTACAACCGGATTAGATACCCTGGTAGTCCACGCCGTAAACGATGAAT150.29007928833881264No Hit
GACTACAACAGGATNAGATACCCTGGTAGTCCACGCCGTAAACGATGTCA140.2707406691162251No Hit
GACTACAACAGGATTAGATACCCTGGTAGTCCACGCCGTACACGATGAGT120.2320634306710501No Hit
GACTACAACCGGATTAGATACCCTGGTAGTCCACGCCGTACACGATGAGT120.2320634306710501No Hit
GACTACACAGGATTAGATACCCTGGTAGTCCACGCCGTAAACGATGAGTG120.2320634306710501No Hit
GACTACAACAGGAGTAGATACCCTGGTAGTCCACGCCGTAAACGATGAGT100.19338619222587508No Hit
GACTACAACAGGATTAGATACCCTGGTAGTCCACGCCGTAAACGATGTAA90.17404757300328758No Hit
GACTACAACAGGATTAGATACCCTGGTAGTCCACGCCGTAAACGATGATA90.17404757300328758No Hit
GACTACAACCGGATTAGATACCCTGGTAGTCCACGCCGTAAACGAGGTAA80.15470895378070004No Hit
GACTACAACCGGATTAGATACCCTGGTAGTCCACGCCGTAAACGAGGAGT70.13537033455811254No Hit
GACTACAACCGGTTAGATACCCTGGTAGTCCACGCCGTAAACGATGAGTG70.13537033455811254No Hit
GACTACAACCGGATTAGATACCCTGGTAGTCCACGCCGTAAACGATGTAA70.13537033455811254No Hit
GACTACACCGGATTAGATACCCTGGTAGTCCACGCCGTAAACGATGAGTG70.13537033455811254No Hit
GACTCAACAGGATTAGATACCCTGGTAGTCCACGCCGTAAACGATGAGTG60.11603171533552505No Hit
GACTACAACCGGATTAGCTACCCTGGTAGTCCACGCCGTAAACGATGAGT60.11603171533552505No Hit
GACTACAACAGGATNAGATACCCTGGTAGTCCACGCCGTAAACGATGATA60.11603171533552505No Hit
GACTACAACAGGATTAGATACCCTGGTAGTCCACGCCGTAAACGATGTTA60.11603171533552505No Hit
GACTACAACCGGATTAGATACACTGGTAGTCCACGCCGTAAACGATGAGT60.11603171533552505No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)