FastQCFastQC Report
Mon 17 Apr 2023
000000000-KWF5G_l01_n01_ELP2G4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-KWF5G_l01_n01_ELP2G4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2755
Sequences flagged as poor quality0
Sequence length301
%GC53

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GACTACAACCGGATTAGATACCCTGGTAGTCCACGCCGTAAACGATGAGT112040.653357531760435No Hit
GACTACAACAGGATTAGATACCCTGGTAGTCCACGCCGTAAACGATGAGT99236.00725952813067No Hit
GACTACAACAGGATTAGATACCCTGGTAGTCCACGCCGTAAACGATGTCA833.012704174228675No Hit
GACTACAACCGGATTAGATACCCTGGTAGTCCACGCCGTAAACGATGTCA792.867513611615245No Hit
GACTACAACCGGATNAGATACCCTGGTAGTCCACGCCGTAAACGATGAGT291.0526315789473684No Hit
GACTACAACAGGATTAGATACCCTGGTAGTCCACGCCCTAAACGATGTCA240.8711433756805809No Hit
GACTACAACAGGATNAGATACCCTGGTAGTCCACGCCGTAAACGATGAGT230.8348457350272231No Hit
GACTACAACCGGATTAGATACCCTGGTAGTCCACGCCGTAAACGATGAAT180.6533575317604355No Hit
GACTACAACAGGATTAGATACCCTGGTAGTCCACGCCGTAAACGATGAAT170.617059891107078No Hit
GACTACAACCGGATTAGATACCCTGGTAGTCCACGCCCTAAACGATGTCA140.5081669691470054No Hit
GACTACAACCGGATTAGATACCCTGGTAGTCCACGCCGTACACGATGAGT140.5081669691470054No Hit
GACTACACCGGATTAGATACCCTGGTAGTCCACGCCGTAAACGATGAGTG80.2903811252268602No Hit
GACTACAACCGGATTAGATACACTGGTAGTCCACGCCGTAAACGATGAGT70.2540834845735027No Hit
GACTACAACAGGATTAGATACCCTGGTAGTCCACGCCGTACACGATGAGT60.21778584392014522No Hit
GACTACAACAGGATTAGATACCATGGTAGTCCACGCCGTAAACGATGAGT60.21778584392014522No Hit
GACTACAACAGGAGTAGATACCCTGGTAGTCCACGCCGTAAACGATGAGT50.18148820326678766No Hit
GACTCAACCGGATTAGATACCCTGGTAGTCCACGCCGTAAACGATGAGTG50.18148820326678766No Hit
GACTACAACAGGATTAGATACCCTGGTAGTCCACGCCGTAAACGATGATT50.18148820326678766No Hit
GACTACAACAGGATTAGATACCCTGGTAGTCCACGCCGTCAACGATGAGT50.18148820326678766No Hit
GACTACAACCGGATTAGATACCCTGGTAGTCCACGCCGTAAACGAGGAGT40.1451905626134301No Hit
GACTACAAACCGGATTAGATACCCTGGTAGTCCACGCCGTAAACGATGAG40.1451905626134301No Hit
GACTACAAACGGATTAGATACCCTGGTAGTCCACGCCGTAAACGATGAGT40.1451905626134301No Hit
GACTACAACAGGACTAGATACCCTGGTAGTCCACGCCGTAAACGATGAGT40.1451905626134301No Hit
GACTACAACCGGATTGATACCCTGGTAGTCCACGCCGTAAACGATGAGTG40.1451905626134301No Hit
GACTACAACCGGATTAGATACCCTGGTATTCCACGCCGTAAACGATGAGT30.10889292196007261No Hit
GACTACAACCGGATTAGATACCCTGGTAGTCCACGCCGTAAACGATGTGT30.10889292196007261No Hit
GACTACAACCGGATTAGATACCCTGGTAGTCCACGCCGTAAACGATGCGT30.10889292196007261No Hit
GACTACAACAGGATTAGATACCCTGGTAGTTCACGCCGTAAACGATGAGT30.10889292196007261No Hit
GACTACAACAGGAGTAGAGACCCGGGTAGGCCACGCCGTAAACGAGGAGT30.10889292196007261No Hit
GACTACACAGGATTAGATACCCTGGTAGTCCACGCCGTAAACGATGAGTG30.10889292196007261No Hit
GACTACAACCGGATTAGATACCCTGGTAGTCCACGCCGTAAAAGATGAGT30.10889292196007261No Hit
GACTACAACCGGAGTAGATACCCTGGTAGTCCACGCCGTAAACGATGAGT30.10889292196007261No Hit
GACTACACCAGGATTAGATACCCTGGTAGTCCACGCCGTAAACGATGAGT30.10889292196007261No Hit
GACTACAACCGGCTTAGATACCCTGGTAGTCCACGCCGTAAACGATGAGT30.10889292196007261No Hit
GACTACAACCGGATTAGATACCCTGGTAGTCCACGCCGTAAACGATGAGA30.10889292196007261No Hit
GACTACAACAGGATTAGATACCCTGGTAGTCCACGCCGTAAACGATGTGT30.10889292196007261No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)