FastQCFastQC Report
Mon 17 Apr 2023
000000000-KWF5G_l01_n01_ELP2F9.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-KWF5G_l01_n01_ELP2F9.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5971
Sequences flagged as poor quality0
Sequence length301
%GC50

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GACTACAACCGGATTAGATACCCTGGTAGTCCACGCCCTAAACGATGTCA281347.11103667727349No Hit
GACTACAACAGGATTAGATACCCTGGTAGTCCACGCCCTAAACGATGTCA227938.16781108692011No Hit
GACTACAACCGGATNAGATACCCTGGTAGTCCACGCCCTAAACGATGTCA801.3398090772064981No Hit
GACTACAACAGGATNAGATACCCTGGTAGTCCACGCCCTAAACGATGTCA550.9211187405794675No Hit
GACTACAACCGGATTAGATACCCTGGTAGTCCACGCCCTACACGATGTCA390.6531569251381678No Hit
GACTACAACAGGATTAGATACCCTGGTAGTCCACGCCCTACACGATGTCA310.519176017417518No Hit
GACTACAACAGGATTAGATACCCTGGTAGTCCACGCACTAAACGATGTCA220.36844749623178696No Hit
GACTACAACCGGATTAGATACCCTGGTAGTCCACGCACTAAACGATGTCA200.33495226930162453No Hit
GACTACAACCGGATTAGATACCCTGGTAGTCCACGCCCTAAACGATGTTA170.28470942890638085No Hit
GACTACAACAGGATTAGATACCCTGGTAGTCCACGCCATAAACGATGTCA160.2679618154412996No Hit
GACTACAACCGGATTAGATACCCTGGTAGTCCACGCCATAAACGATGTCA130.21771897504605595No Hit
GACTCAACAGGATTAGATACCCTGGTAGTCCACGCCCTAAACGATGTCAA110.18422374811589348No Hit
GACTACACAGGATTAGATACCCTGGTAGTCCACGCCCTAAACGATGTCAA110.18422374811589348No Hit
GACTCAACCGGATTAGATACCCTGGTAGTCCACGCCCTAAACGATGTCAA110.18422374811589348No Hit
GACTACAACCGGATTAGCTACCCTGGTAGTCCACGCCCTAAACGATGTCA100.16747613465081226No Hit
GACTACAACAGGATTAGATACCCTGGTAGTCCACGCGCTAAACGATGTCA90.15072852118573102No Hit
GACTACAACCGGATTAGATACCCTGGTAGTCCACGCCCTAAACGATGTCT90.15072852118573102No Hit
GACTACAACAGGATTAGATACCCTGGTAGTCCACGCCCTAAACGATGTCT90.15072852118573102No Hit
GACTACACCGGATTAGATACCCTGGTAGTCCACGCCCTAAACGATGTCAA90.15072852118573102No Hit
GACTACAACCGGATTAGATACCCTGGTAGTCCACGCCCTCAACGATGTCA80.1339809077206498No Hit
GACTACAACCGGATTAGATACCCTGGTAGTCCACGCCCTAAACGATGTAA80.1339809077206498No Hit
GACTACAACCGGATTAGATACCATGGTAGTCCACGCCCTAAACGATGTCA80.1339809077206498No Hit
GACTACACCAGGATTAGATACCCTGGTAGTCCACGCCCTAAACGATGTCA70.11723329425556857No Hit
GACTACAACAGGATTAGCTACCCTGGTAGTCCACGCCCTAAACGATGTCA70.11723329425556857No Hit
GACTACAACAGGATTAGATACCATGGTAGTCCACGCCCTAAACGATGTCA70.11723329425556857No Hit
GACTACAACCGGATTAGATACACTGGTAGTCCACGCCCTAAACGATGTCA70.11723329425556857No Hit
GACTACAACAGGATTAGATACCCTGGTAGTCCACGCCCTAAACGATGTTA70.11723329425556857No Hit
GACTACAACCGGATTAGATACCCTGGTAGTCCACGCGCTAAACGATGTCA70.11723329425556857No Hit
GACTACAACAGGATTCGATACCCTGGTAGTCCACGCCCTAAACGATGTCA70.11723329425556857No Hit
GACTACAACAGGATTAGATACCCTGGTAGTCCACGCCGTAAACGATGTCA70.11723329425556857No Hit
GACTACAACCGGATTAGATACCCTGGTAGTTCACGCCCTAAACGATGTCA60.10048568079048736No Hit
GACTACAACCGGATTAGATACCCTGGTAGTACACGCCCTAAACGATGTCA60.10048568079048736No Hit
GACTACAACCGGATTAGATCCCCTGGTAGTCCACGCCCTAAACGATGTCA60.10048568079048736No Hit
GACTACACCCGGATTAGATACCCTGGTAGTCCACGCCCTAAACGATGTCA60.10048568079048736No Hit
GACTACAACCGGATTAGATACCCTGGTAGTCCACGCCCTAAACGATATCA60.10048568079048736No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)