FastQCFastQC Report
Mon 17 Apr 2023
000000000-KWF5G_l01_n01_ELP2F5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-KWF5G_l01_n01_ELP2F5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences57
Sequences flagged as poor quality0
Sequence length301
%GC51

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GACTACAACAGGATTAGATACCCTGGTAGTCCACGCCGTAAACGATGTCA2442.10526315789473No Hit
GACTACAACCGGATTAGATACCCTGGTAGTCCACGCCGTAAACGATGTCA1831.57894736842105No Hit
GACTACAACAGGATTAGATACCCTGGTAGTCCACGCCGTAAACGATGAGT35.263157894736842No Hit
GACTACAACCGGATTAGATACCCTGGTAGTCCACGCCGTAAACGATGATA23.508771929824561No Hit
GACCACAACAGGATTAGATACCCTGGTAGTCCACGCCGTAAACGATGTCA23.508771929824561No Hit
GACTACAACAGGATTAGATCCCCTGGTAGTCCACGCCGTAAACGATGTCA11.7543859649122806No Hit
GACTACAACAGGATTAGATACCCTGGTAGTCCACGCCGTAAACGATGTAA11.7543859649122806No Hit
GACTACAACCGGATTAGATACCATGGTAGTCCACGCCGTAAACGATGAGT11.7543859649122806No Hit
GACTACAACCGGATTAGATACCCTGGTAGTCCACGCAGTAAACGATGTCA11.7543859649122806No Hit
GACTACAACCGGATTAGATACCCTGGTAGTCCACGCCGTCAACGATGTCA11.7543859649122806No Hit
GACTACAACAGGATTAGATACCCTGGTAGTCCACGCCGTCAACGATGTCA11.7543859649122806No Hit
GACTACAACAGGATTAGATACCCTGGTAGTCCACGCCCTAAACGATGTCA11.7543859649122806No Hit
GACTACAACAGGATTAGATACCCTGGTAGTCCACGCCGTACACGATGTCA11.7543859649122806No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)