FastQCFastQC Report
Mon 17 Apr 2023
000000000-KWF5G_l01_n01_ELP2F3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-KWF5G_l01_n01_ELP2F3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1238
Sequences flagged as poor quality0
Sequence length301
%GC51

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GACTACAACCGGATTAGATACCCTGGTAGTCCACGCCCTAAACGATGTCA59848.30371567043619No Hit
GACTACAACAGGATTAGATACCCTGGTAGTCCACGCCCTAAACGATGTCA47838.61066235864297No Hit
GACTACAACCGGATNAGATACCCTGGTAGTCCACGCCCTAAACGATGTCA120.9693053311793215No Hit
GACTACAACAGGATTAGATACCCTGGTAGTCCACGCCGTAAACGATGTCA120.9693053311793215No Hit
GACTACAACCGGATTAGATACCCTGGTAGTCCACGCCGTAAACGATGTCA80.6462035541195477No Hit
GACTACAACCGGATTAGATACCCTGGTAGTCCACGCCCTACACGATGTCA80.6462035541195477No Hit
GACTACAACAGGATNAGATACCCTGGTAGTCCACGCCCTAAACGATGTCA50.40387722132471726No Hit
GACTACAACAGGATTAGATACCCTGGTAGTCCACGCCCTACACGATGTCA40.32310177705977383No Hit
GACTACAACCGGATTAGATACCCTGGTAGTCCACGCGCTAAACGATGTCA40.32310177705977383No Hit
GACTACACCCGGATTAGATACCCTGGTAGTCCACGCCCTAAACGATGTCA40.32310177705977383No Hit
GACTACAACCGGATTAGCTACCCTGGTAGTCCACGCCCTAAACGATGTCA30.24232633279483037No Hit
GACTACAACAGGATTAGATACCCTGGTAGTCCACGCCCTAAACGATGTTA30.24232633279483037No Hit
GACTACAACAGGATTAGATACCCTGATAGTCCACGCCCTAAACGATGTCA30.24232633279483037No Hit
GACTACAACCGGACTAGATACCCTGGTAGTCCACGCCCTAAACGATGTCA30.24232633279483037No Hit
GACTACAAACCGGATTAGACACCCTGGTAGTCCACGCCCTAAACGATGTC30.24232633279483037No Hit
GACTACAACAGGATTAGATACCCTGGTAGTCCACGCCATAAACGATGTCA20.16155088852988692No Hit
GACTACAACAGGATTGATACCCTGGTAGTCCACGCCCTAAACGATGTCAA20.16155088852988692No Hit
GACTACAACAGGATTAGATACCCTGGTAGTCCACGCGCTAAACGATGTCA20.16155088852988692No Hit
GACTACAACCGGATCAGATACCCTGGTAGTCCACGCCCTAAACGATGTCA20.16155088852988692No Hit
GACTACAACCGGATTAGATACCCTGGTAGTCTACGCCCTAAACGATGTCA20.16155088852988692No Hit
GACTACAACCGGATTAATACCCTGGTAGTCCACGCCCTAAACGATGTCAA20.16155088852988692No Hit
GACTACAACCGGATTAGATACCCTGGTAGTCCACGCCATAAACGATGTCA20.16155088852988692No Hit
GACTACAACAGGATTAGCTACCCTGGTAGTCCACGCCCTAAACGATGTCA20.16155088852988692No Hit
GACTACAACCGGATTGATACCCTGGTAGTCCACGCCCTAAACGATGTCAA20.16155088852988692No Hit
GGACTACAACCGGATTAGATACCCTGGTAGTCCACGCCCTAAACGATGTC20.16155088852988692No Hit
GACTACAACCGGATTAGATACCCTGGTAGTCCACGCCCTAAACGATGTCT20.16155088852988692No Hit
GCTACAACCGGATTAGATACCCTGGTAGTCCACGCCCTAAACGATGTCAA20.16155088852988692No Hit
GACTACAACAGGATTAGATACCCTGGTAGTCCACGCCCTCAACGATGTCA20.16155088852988692No Hit
GACTCAACAGGATTAGATACCCTGGTAGTCCACGCCCTAAACGATGTCAA20.16155088852988692No Hit
GACTACAACCGGATTAGATACCCTGGTAGTCCACGCCCTAAACTATGTCA20.16155088852988692No Hit
GACTACAACAGGGATTAGATACCCTGGTAGTCCACGCCGTAAACGATGAG20.16155088852988692No Hit
GACTACAACCGATTAGATACCCTGGTAGTCCACGCCCTAAACGATGTCAA20.16155088852988692No Hit
GACTACAACCGGTTAGATACCCTGGTAGTCCACGCCCTAAACGATGTCAA20.16155088852988692No Hit
GACTACACAGGATTAGATACCCTGGTAGTCCACGCCCTAAACGATGTCAA20.16155088852988692No Hit
GACTACACCGGATTAGATACCCTGGTAGTCCACGCCCTAAACGATGTCAA20.16155088852988692No Hit
GACTACAACAGGATTAGATACCCTGGTAGTCCACGCCCTAAGCGATGTCA20.16155088852988692No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)